English H F, Schanbacher B D, Gross D, Walker M F, Falvo R E, Santen R J
J Androl. 1983 Jul-Aug;4(4):240-7. doi: 10.1002/j.1939-4640.1983.tb02360.x.
Morphologic changes in the male reproductive system of mongrel dogs immunized against LHRH were quantitated using linear measurements and morphometric techniques at the light-microscopic level. Two experimental groups (5 nonimmunized control animals and 5 actively immunized animals) were killed 12 weeks after the primary immunization. No significant differences were observed between three immunized dogs having low LHRH antibody titers (immunized-unaffected) and the five nonimmunized control dogs. The two immunized dogs (affected) with the highest antibody titers against LHRH were characterized by atrophy and dedifferentiation of the testes, prostate, and excurrent ducts. The morphologic changes in the testes of these two dogs were striking and included an apparent arrest or significant reduction in the spermatogenic process, concurrent epithelial degeneration, and apparent diminution of Leydig cell mass. Drastic reductions in the size of the prostatic acini and epithelial cells, as well as loss of secretory granules, reflected depression of function and androgen production. Similarly, in the excurrent ducts decreases in the measured parameters and loss of regional cytoplasmic specialization denoted functional decrescence. This study demonstrates the regressive effects of LHRH immunoneutralization on the morphology of the reproductive system in the male dog and further supports the feasibility of this system as an animal model for the study of isolated gonadotropin deficiency.
采用线性测量和形态计量学技术在光学显微镜水平对免疫促黄体生成素释放激素（LHRH）的杂种犬雄性生殖系统的形态学变化进行定量分析。初次免疫12周后处死两个实验组（5只未免疫对照动物和5只主动免疫动物）。LHRH抗体效价低的3只免疫犬（免疫未受影响）与5只未免疫对照犬之间未观察到显著差异。两只LHRH抗体效价最高的免疫犬（受影响）的特征为睾丸、前列腺和输出管萎缩及去分化。这两只犬睾丸的形态学变化显著，包括生精过程明显停滞或显著减少、同时伴有上皮细胞变性以及间质细胞数量明显减少。前列腺腺泡和上皮细胞大小急剧减小以及分泌颗粒丢失，反映了功能和雄激素产生的抑制。同样，在输出管中，测量参数降低以及局部细胞质特化丧失表明功能减退。本研究证明了LHRH免疫中和对雄性犬生殖系统形态的退行性影响，并进一步支持了该系统作为研究孤立性促性腺激素缺乏动物模型的可行性。
