Cavenagh M M, Miller R V
J Bacteriol. 1986 Feb;165(2):448-52. doi: 10.1128/jb.165.2.448-452.1986.
D3, a temperate bacteriophage of Pseudomonas aeruginosa PAO, was found to specifically transduce the alleles met-49 and met-117. Induction of established lysogens with UV light was necessary for the production of transducing lysates. Transduced cells were immune to superinfection by phage D3 and could give rise to high-frequency transducing lysates. Cotransduction of these two alleles could not be demonstrated. met-117 was mapped to 26 min on the PAO genetic map. Complementation studies using the generalized transducing phage F116L indicated that met-49 is an allele of met-9011 which maps at 55 min. The integrated D3 prophage was shown to be coinherited with met-117 and with met-49.
铜绿假单胞菌PAO的温和噬菌体D3被发现能特异性转导met - 49和met - 117等位基因。用紫外线诱导已建立的溶原菌对于产生转导裂解物是必要的。转导细胞对噬菌体D3的超感染具有免疫性，并且能够产生高频转导裂解物。这两个等位基因的共转导无法得到证实。met - 117被定位在PAO遗传图谱的26分钟处。使用广义转导噬菌体F116L进行的互补研究表明，met - 49是位于55分钟处的met - 9011的一个等位基因。整合的D3原噬菌体被证明与met - 117和met - 49共同遗传。
