Warner M R
J Endocrinol. 1978 Apr;77(1):1-10. doi: 10.1677/joe.0.0770001.
Morphological differentiation was enhanced in mammary glands from mice injected with oestradiol as neonates both in vivo, after 9 days of pretreatment with oestrogen and progesterone, and in vitro, either after 6 or 9 days of pretreatment and on all hormone supplements, or after culture with oestradiol, progesterone, aldosterone, insulin and thyroxine with or without prolactin and growth hormone. These hormone combinations further enhanced development, whereas culture with insulin and thyroxine did not. Differentiation in vitro was least on medium containing insulin + thyroxine, and was greatest on the complete medium (insulin, thyroxine, growth hormone, prolactin, aldosterone, progesterone and oestradiol). An intermediate degree of differentiation occurred when growth hormone and prolactin were omitted from the complete medium. Differences in lobule formation between oestrogen-treated and control mice were probably ovary-dependent at the dose used (25 microgram/day), since pretreatment with oestradiol and progesterone was necessary to reveal them. Enhanced hormone sensitivity of murine mammary tissues exposed to oestradiol early in life may be related to subsequent enhanced dysplastic potential of the tissue and provides a further basis for caution in the administration of steroids to immature individuals.
新生小鼠注射雌二醇后，其乳腺的形态分化在体内和体外均得到增强。在体内，经雌激素和孕酮预处理9天后；在体外，经6天或9天预处理且添加所有激素补充剂后，或者在用雌二醇、孕酮、醛固酮、胰岛素和甲状腺素培养（添加或不添加催乳素和生长激素）后，均出现这种情况。这些激素组合进一步促进了乳腺发育，而仅用胰岛素和甲状腺素培养则无此效果。体外分化在含胰岛素+甲状腺素的培养基上程度最低，在完全培养基（胰岛素、甲状腺素、生长激素、催乳素、醛固酮、孕酮和雌二醇）上程度最高。当完全培养基中省略生长激素和催乳素时，会出现中等程度的分化。在所使用的剂量（25微克/天）下，雌激素处理组和对照组小鼠在小叶形成上的差异可能依赖于卵巢，因为必须用雌二醇和孕酮预处理才能显现出这些差异。生命早期接触雌二醇的小鼠乳腺组织对激素的敏感性增强，可能与该组织随后发育异常的可能性增加有关，并为对未成熟个体使用类固醇时需谨慎提供了进一步的依据。
