Ohori H, Kanno A, Nagatsuka Y, Yamada E, Onodera S, Tateda A, Abe Y, Togoh T, Ishida N
J Med Virol. 1983;12(3):161-78. doi: 10.1002/jmv.1890120302.
The antigen/antibody systems specific for non-A, non-B hepatitis (NANB) were studied using urine samples as the antigen source and sera for the antibody source. Two immunologically distinct systems, SO-antigen/anti-SO and MI-antigen/anti-MI were discovered. This paper deals chiefly with the characterization of the SO-antigen, which was associated with an epidemic-type non-A, non-B hepatitis found during September 1979 to February 1980 (first outbreak) and October 1980 to January 1981 (second outbreak) in the Cardiovascular Surgical Unit of Tohoku University Hospital. All patients who developed non-A, non-B hepatitis during the first and second epidemic periods had SO-antigen in their urine (24 out of 24). After the epidemic, however, the detection rate of SO-antigen gradually decreased among patients in the same unit, although posttransfusion non-A, non-B hepatitis continued to be found. The final detection of SO-antigen occurred at or just after the elevation of alanine aminotransferase (ALT) levels during the episode of hepatitis and persisted in most cases throughout the elevated period. Anti-SO antibody was detected relatively late (eight months after blood transfusion, in most cases) and apparently persisted longer than five years. The immunological and physicochemical properties of SO-antigen were also studied. It appeared to be neither a plasma protein nor a liver tissue component when the cross-reactivity of SO-antigen was examined by the immunodiffusion method. Absorption with insolubilized human serum and liver tissues failed to affect the anti-SO antibody activity. The molecular weight of SO-antigen was estimated to be 250,000, the sedimentation coefficient to be 11.0 S, and the buoyant density in CsCl to be 1.215 g/cm3. Electron microscopy showed that the SO-antigen corresponded with uniform particles with a mean diameter of 11 nm. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of SO-antigen revealed only a single protein band corresponding to molecular weight 38,000.
以尿液样本作为抗原来源、血清作为抗体来源，对非甲非乙型肝炎（NANB）特异性的抗原/抗体系统进行了研究。发现了两个免疫上不同的系统，即SO抗原/抗SO和MI抗原/抗MI。本文主要论述SO抗原的特性，该抗原与1979年9月至1980年2月（首次暴发）以及1980年10月至1981年1月（第二次暴发）在东北大学医院心血管外科病房发现的流行性非甲非乙型肝炎有关。在第一次和第二次流行期间发生非甲非乙型肝炎的所有患者尿液中均有SO抗原（24例患者全部如此）。然而，流行过后，同一病房患者中SO抗原的检出率逐渐下降，尽管仍不断发现输血后非甲非乙型肝炎病例。SO抗原的最后检出发生在肝炎发作期间丙氨酸转氨酶（ALT）水平升高之时或之后不久，并且在大多数情况下在升高期一直存在。抗SO抗体相对较晚才被检测到（大多数情况下在输血后8个月），而且显然持续存在超过5年。还研究了SO抗原的免疫学和物理化学特性。当通过免疫扩散法检测SO抗原的交叉反应性时，它似乎既不是血浆蛋白也不是肝组织成分。用人血清和肝组织的不溶性制剂进行吸收，并不影响抗SO抗体活性。估计SO抗原的分子量为250,000，沉降系数为11.0 S，在氯化铯中的浮力密度为1.215 g/cm3。电子显微镜检查显示，SO抗原与平均直径为11 nm的均匀颗粒相对应。对SO抗原进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析，仅显示出一条对应于分子量38,000的单一蛋白带。
