Spertini O, Frei P C
Med Microbiol Immunol. 1986;175(4):229-39. doi: 10.1007/BF02123731.
The antigen from a non-A, non-B antigen-antibody system previously described was purified and, when tested by immunodiffusion, was shown to produce patterns of identity with all antigen-positive sera of the system. The sedimentation coefficient S20,w of the antigen was estimated by rate zonal ultracentrifugation to be 48. Isopycnic ultracentrifugation in CsCl gradient of non-A, non-B antigen generated a single sedimentation peak at the density of 1.28. SDS-polyacrylamide-agarose gel electrophoresis showed that I125-labelled antigen migrated as a single band (molecular weight 3.0 X 10(6)); in addition, labelled material also migrated to the albumin region. With SDS-PAGE under reducing conditions, the antigen was shown to consist of seven polypeptides. Spherical particles of 23 nm in diameter, demonstrated by electron microscopy, could be aggregated by purified non-A, non-B antibody. They probably represent the antigen and not a complete viral particle. The characteristics of this antigen associated with non-A, non-B hepatitis appear therefore to be close to those of hepatitis B surface antigen.
先前描述的非甲非乙型抗原抗体系统中的抗原被纯化，经免疫扩散试验表明，它与该系统所有抗原阳性血清产生同一反应模式。通过速率区带超速离心法估计该抗原的沉降系数S20,w为48。非甲非乙型抗原在CsCl梯度中的等密度超速离心在密度1.28处产生一个单一沉降峰。SDS-聚丙烯酰胺-琼脂糖凝胶电泳显示，I125标记的抗原迁移为单一区带（分子量3.0×10(6)）；此外，标记物质也迁移至白蛋白区带。在还原条件下进行SDS-PAGE时，该抗原显示由七条多肽组成。电子显微镜显示直径为23nm的球形颗粒可被纯化的非甲非乙型抗体凝集。它们可能代表抗原而非完整病毒颗粒。因此，与非甲非乙型肝炎相关的这种抗原的特性似乎与乙型肝炎表面抗原的特性相近。
