Stafford J, Queen C
Nature. 1983;306(5938):77-9. doi: 10.1038/306077a0.
The introduction of cloned genes into eukaryotic cells has become a major technique in the study of gene expression. Many experiments have demonstrated transcription of cloned genes after transfection into heterologous cell systems--cells in which the genes are not normally active. More recently, several investigators have obtained expression of specialized genes after transfection into cells of the corresponding specialized type, notably the beta-globin gene in erythroleukaemic cells and immunoglobulin genes in myeloma cells. These results allow the study of gene expression during development by comparing transcription of a gene transfected into homologous and heterologous cells. We have shown that a rearranged kappa immunoglobulin gene, cloned from a mouse myeloma, is transcribed transiently at a high level when reintroduced into mouse myeloma cells. We show here, in an internally controlled manner, that the same immunoglobulin gene is not detectably transcribed when transfected into mouse 3T3 or L cells.
将克隆基因导入真核细胞已成为基因表达研究中的一项主要技术。许多实验表明，将克隆基因转染到异源细胞系统（即基因通常不活跃的细胞）后，这些基因能够转录。最近，一些研究人员在将特定基因转染到相应的特定类型细胞后，实现了这些基因的表达，尤其是将β - 珠蛋白基因转染到红白血病细胞中，以及将免疫球蛋白基因转染到骨髓瘤细胞中。这些结果使得通过比较转染到同源细胞和异源细胞中的基因转录情况，来研究发育过程中的基因表达成为可能。我们已经表明，从小鼠骨髓瘤中克隆得到的重排κ免疫球蛋白基因，当重新导入小鼠骨髓瘤细胞时会短暂地高水平转录。我们在此以一种内部控制的方式表明，当该免疫球蛋白基因转染到小鼠3T3或L细胞中时，未检测到可察觉的转录。
