Gavaler J S, Perez H A, Estes L, Van Thiel D H
Pharmacol Biochem Behav. 1983;18 Suppl 1:341-7. doi: 10.1016/0091-3057(83)90197-1.
Ultrastructural morphometric studies were employed to compare the morphology of Leydig cells found in the testes of rats fed ethanol, accounting for 36% of total calories for 6 weeks, and those found in the testes of rats isocalorically fed a diet in which ethanol was isocalorically replaced with dextrimaltose. The testes of alcohol-fed rats weighed significantly less (p less than 0.01) than those of the isocaloric controls. Moreover, plasma testosterone in the alcohol-fed animals was reduced (p less than 0.01), as compared to the controls. Morphologically, two main differences were encountered between the Leydig cells of alcohol-fed animals and those of the isocalorically-fed controls. These were: (1) an increase in the number of elongated and cup-shaped mitochondria; and (2) an increase in cytoplasmic protrusions in the form of pseudopods. Using morphometric techniques, the Leydig cells of alcohol-fed animals were smaller (p less than 0.05), had less cytoplasm (p less than 0.05), larger mitochondria (p less than 0.01), and less smooth endoplasmic reticulum (p less than 0.05) than did those of the isocaloric controls. These morphologic characteristics of Leydig cells of alcohol-fed animals are similar to those reported to occur in the liver and suggest that the biochemical mechanisms responsible for alcohol-induced cellular injury are similar in the testes and the liver.
采用超微结构形态计量学研究方法，比较了两组大鼠睾丸中Leydig细胞的形态。一组大鼠摄入乙醇6周，乙醇热量占总热量的36%；另一组大鼠摄入等热量饮食，其中乙醇被等热量的糊精麦芽糖替代。摄入酒精的大鼠睾丸重量显著低于等热量对照组（p<0.01）。此外，与对照组相比，摄入酒精动物的血浆睾酮水平降低（p<0.01）。在形态学上，摄入酒精动物的Leydig细胞与等热量喂养对照组的Leydig细胞存在两个主要差异。这些差异是：（1）细长形和杯状线粒体数量增加；（2）以伪足形式存在的细胞质突起增加。使用形态计量学技术，摄入酒精动物的Leydig细胞比等热量对照组的细胞更小（p<0.05），细胞质更少（p<0.05），线粒体更大（p<0.01），滑面内质网更少（p<0.05）。摄入酒精动物Leydig细胞的这些形态学特征与肝脏中报道的特征相似，表明睾丸和肝脏中负责酒精诱导细胞损伤的生化机制相似。
