Christner A, Bormann E J, Reiche R
Z Allg Mikrobiol. 1983;23(6):351-8.
Phenotypically different submerged mycelium conserves had been produced from a spore conserve of the HP-strain Streptomyces griseus and proofed in a product formation culture as a test system. The phenotypical characters induced on the base of the genotype proved in a cultivation cycle during 30-34 reduplications of the biomass constant. Employing the HP phenotype we investigated the possibility of economizing the substrate turnover by utilizing the anabolic potential for the synthesis of secondary substances and/or reducing the conservation catabolism during the stationary growth stage. As criteria for that served the stoichiometric turnover equation of the streptomycin biosynthesis and the quotient qO2/qGluc taking at full substrate oxidation the numerical value 6. During the stationary growth stage the relation of maintenance anabolism to maintenance catabolism in addition to the formation as secondary substances is not fixed in the tested HP phenotypes, but in a striking manner variable. The relation of by-product synthesis to secondary metabolism synthesis, too, is variable in the stationary growth stage with constant maintenance catabolism. Due to those response reactions on phenotypical manipulations an economization of the substrate turnover during the product formation stage with stationary growth is not possible in the streptomycin producer Streptomyces griseus.
从灰色链霉菌HP菌株的孢子保存物中产生了表型不同的深层菌丝体保存物，并在作为测试系统的产物形成培养中得到验证。在生物量恒定的30 - 34次复制的培养周期中，基于基因型诱导的表型特征得到了证实。利用HP表型，我们研究了通过利用合成次生物质的合成代谢潜力和/或减少稳定生长阶段的保存分解代谢来节约底物周转的可能性。为此，以链霉素生物合成的化学计量周转方程和在底物完全氧化时qO2/qGluc的商值6作为标准。在稳定生长阶段，除了作为次生物质形成外，测试的HP表型中维持合成代谢与维持分解代谢的关系并不固定，而是显著可变的。在稳定生长阶段，副产物合成与次生代谢合成的关系在维持分解代谢恒定的情况下也是可变的。由于对表型操作的这些反应，在链霉素产生菌灰色链霉菌中，在稳定生长的产物形成阶段节约底物周转是不可能的。
