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产CRM197的白喉棒状杆菌非串联双溶原菌的分离与鉴定

Isolation and characterization of Corynebacterium diphtheriae nontandem double lysogens hyperproducing CRM197.

作者信息

Rappuoli R

出版信息

Appl Environ Microbiol. 1983 Sep;46(3):560-4. doi: 10.1128/aem.46.3.560-564.1983.

DOI:10.1128/aem.46.3.560-564.1983
PMID:6416165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC239316/
Abstract

Phage beta 197tox-, which codes for CRM197, a nontoxic protein immunochemically indistinguishable from diphtheria toxin, was UV induced from a culture of the C7(beta 197)tox- strain. A total of 191 C7(beta 197)tox- lysogens were isolated and selected according to the halo produced on TYE agar containing antidiphtheria toxin serum and were further characterized by Southern blots of their chromosomal DNA. Most of the isolates turned out to be monolysogens, but some tandem and nontandem double lysogens were also found. The nontandem double lysogens were stable and capable of giving high yields of CRM197, up to threefold higher than monolysogens. They are, therefore, suitable for large-scale industrial production.

摘要

噬菌体β197tox-可编码CRM197，一种与白喉毒素在免疫化学上无法区分的无毒蛋白质，它是从C7(β197)tox-菌株的培养物经紫外线诱导产生的。总共分离并筛选出191个C7(β197)tox-溶原菌，依据在含有抗白喉毒素血清的TYE琼脂上产生的晕圈进行选择，并通过对其染色体DNA的Southern杂交进一步鉴定。大多数分离株结果是单溶原菌，但也发现了一些串联和非串联双溶原菌。非串联双溶原菌很稳定，能够高产CRM197，产量比单溶原菌高出三倍。因此，它们适合大规模工业生产。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83c2/239316/bbe00adaf13f/aem00166-0038-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83c2/239316/b7c9f57a1170/aem00166-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83c2/239316/bbe00adaf13f/aem00166-0038-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83c2/239316/b7c9f57a1170/aem00166-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83c2/239316/bbe00adaf13f/aem00166-0038-b.jpg

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