Rappuoli R, Michel J L, Murphy J R
J Virol. 1983 Feb;45(2):524-30. doi: 10.1128/JVI.45.2.524-530.1983.
The toxigenic corynebacteriophage omega tox+ was isolated from the hypertoxigenic Park-Williams no. 8 (PW8) strain of Corynebacterium diphtheriae and compared with the toxigenic corynebacteriophage beta tox+. The physical size and host range of both phages were found to be identical. An endonuclease restriction map of omega tox+ was constructed, and the locations of the cohesive ends (cos), phage attachment site (attP), and the diphtheria tox operon were identified. The genome of omega tox+ was found to differ from that of beta tox+ in three regions. In addition, omega tox+ was shown to be integrated into two nontandem corynebacterial phage attachment sites (attB1, attB2) in the PW8 chromosome. The differences in the restriction endonuclease digestion maps of omega tox+ and beta tox+ and the contribution of double lysogeny are discussed in relation to the hypertoxigenicity of the PW8 strain.
产毒素的棒状噬菌体ωtox⁺是从产毒力极强的白喉棒状杆菌帕克-威廉姆斯8号（PW8）菌株中分离出来的，并与产毒素的棒状噬菌体βtox⁺进行了比较。发现这两种噬菌体的物理大小和宿主范围相同。构建了ωtox⁺的核酸内切酶限制图谱，并确定了粘性末端（cos）、噬菌体附着位点（attP）和白喉毒素操纵子的位置。发现ωtox⁺的基因组在三个区域与βtox⁺的基因组不同。此外，ωtox⁺被证明整合到PW8染色体中的两个非串联棒状杆菌噬菌体附着位点（attB1、attB2）。结合PW8菌株的高毒性，讨论了ωtox⁺和βtox⁺的限制性内切酶消化图谱的差异以及双重溶原性的作用。
