Payne G A, Hagler W M
Appl Environ Microbiol. 1983 Oct;46(4):805-12. doi: 10.1128/aem.46.4.805-812.1983.
Four amino acids were used as sole nitrogen sources or as supplements to ammonium sulfate, and casein and ammonium sulfate were used as sole nitrogen sources to examine their effects on aflatoxin production by Aspergillus parasiticus NRRL 2999 and Aspergillus flavus 3357 grown on synthetic liquid media. In general, when proline, asparagine, casein, and ammonium sulfate were used as sole nitrogen sources, they supported more growth and toxin production than tryptophan or methionine. However, proline stimulated more toxin production per gram of mycelium in stationary cultures than the other nitrogen sources, including the amino acid asparagine, which is generally recognized as supporting good aflatoxin production. The exact responses to individual nitrogen sources were influenced by the species of fungus and whether cultures were stationary or shaken. In shake cultures, but not in stationary cultures, increased growth was generally associated with increased toxin production.
使用四种氨基酸作为唯一氮源或作为硫酸铵的补充物，并使用酪蛋白和硫酸铵作为唯一氮源，以研究它们对寄生曲霉NRRL 2999和黄曲霉3357在合成液体培养基上生长时产生黄曲霉毒素的影响。总体而言，当脯氨酸、天冬酰胺、酪蛋白和硫酸铵用作唯一氮源时，它们比色氨酸或蛋氨酸支持更多的生长和毒素产生。然而，在静止培养物中，脯氨酸每克菌丝体刺激产生的毒素比其他氮源更多，包括通常认为支持良好黄曲霉毒素产生的天冬酰胺氨基酸。对个别氮源的确切反应受真菌种类以及培养物是静止还是振荡的影响。在振荡培养物中，而非静止培养物中，生长增加通常与毒素产生增加相关。
