巴西固氮螺菌对各种碳源的代谢
Metabolism of various carbon sources by Azospirillum brasilense.
作者信息
Westby C A, Cutshall D S, Vigil G V
出版信息
J Bacteriol. 1983 Dec;156(3):1369-72. doi: 10.1128/jb.156.3.1369-1372.1983.
Abstract
Azospirillum brasilense Sp7 and two mutants were examined for 19 carbon metabolism enzymes. The results indicate that this nitrogen fixer uses the Entner-Doudoroff pathway for gluconate dissimilation, lacks a catabolic but has an anabolic Embden-Meyerhof-Parnas hexosephosphate pathway, has amphibolic triosephosphate enzymes, lacks a hexose monophosphate shunt, and has lactate dehydrogenase, malate dehydrogenase, and glycerokinase. The mutants are severely deficient in phosphoglycerate and pyruvate kinase and also have somewhat reduced levels of other carbon enzymes.
摘要
对巴西固氮螺菌Sp7及其两个突变体进行了19种碳代谢酶的检测。结果表明，这种固氮菌利用恩特纳-杜德洛夫途径进行葡萄糖酸盐异化，缺乏分解代谢途径但具有合成代谢的糖酵解途径，具有兼性的磷酸丙糖酶，缺乏磷酸戊糖途径，并且具有乳酸脱氢酶、苹果酸脱氢酶和甘油激酶。这些突变体在磷酸甘油酸激酶和丙酮酸激酶方面严重缺乏，其他碳代谢酶的水平也有所降低。
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