酿酒酵母糖酵解途径突变体的分离与鉴定
Isolation and characterization of Saccharomyces cerevisiae glycolytic pathway mutants.
作者信息
Lam K B, Marmur J
出版信息
J Bacteriol. 1977 May;130(2):746-9. doi: 10.1128/jb.130.2.746-749.1977.
Abstract
Yeast strains carrying recessive mutations representing four different loci that cause defects in pyruvate kinase, pyruvate decarboxylase, 3-phosphoglycerate kinase, and 3-phosphoglycerate mutase were isolated and partially characterized. Cells carrying these mutations were unable to use glucose as a carbon source as measured in turbidimetric growth experiments. Tetrad analysis indicated that these mutations were not linked to each other; one of the mutations, that affecting phosphoglycerate kinase, was located on chromosome III.
摘要
分离出了携带隐性突变的酵母菌株，这些突变代表了四个不同的基因座，它们分别导致丙酮酸激酶、丙酮酸脱羧酶、3-磷酸甘油酸激酶和3-磷酸甘油酸变位酶出现缺陷，并对其进行了部分表征。在比浊生长实验中，携带这些突变的细胞无法将葡萄糖用作碳源。四分体分析表明，这些突变彼此不连锁；其中一个影响磷酸甘油酸激酶的突变位于第三条染色体上。
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本文引用的文献
1
Biochemical Mutants in the Smut Fungus Ustilago Maydis.
Genetics. 1949 Sep;34(5):607-26. doi: 10.1093/genetics/34.5.607.
2
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
3
Use of snail digestive juice in isolation of yeast spore tetrads.
J Bacteriol. 1959 Aug;78(2):292. doi: 10.1128/jb.78.2.292-292.1959.
4
Molecular weight and coenzyme content of pyruvate decarboxylase from brewer's yeast.
Biochim Biophys Acta. 1966 Mar 7;113(3):595-604. doi: 10.1016/s0926-6593(66)80017-6.
5
A kinetic study of glycolytic enzyme synthesis in yeast.
J Biol Chem. 1971 Jan 25;246(2):475-88.
6
Purification and crystallization of yeast pyruvate kinase.
Hoppe Seylers Z Physiol Chem. 1972 Mar;353(3):435-40. doi: 10.1515/bchm2.1972.353.1.435.
7
Carbohydrate metabolism in microorganisms.
Annu Rev Microbiol. 1969;23:539-78. doi: 10.1146/annurev.mi.23.100169.002543.
8
Isolation and characterization of Escherichia coli mutants defective in enzymes of glycolysis.
Biochem Biophys Res Commun. 1974 Jan;56(1):127-33. doi: 10.1016/s0006-291x(74)80324-4.
9
A revised preparation of yeast (Saccharomyces cerevisiae) pyruvate kinase.
J Biol Chem. 1976 Jan 10;251(1):124-8.
10
Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure.
Biochim Biophys Acta. 1975 Oct 20;405(2):492-9.
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