酿酒酵母糖酵解途径突变体的分离与鉴定
Isolation and characterization of Saccharomyces cerevisiae glycolytic pathway mutants.
作者信息
Lam K B, Marmur J
出版信息
J Bacteriol. 1977 May;130(2):746-9. doi: 10.1128/jb.130.2.746-749.1977.
Abstract
Yeast strains carrying recessive mutations representing four different loci that cause defects in pyruvate kinase, pyruvate decarboxylase, 3-phosphoglycerate kinase, and 3-phosphoglycerate mutase were isolated and partially characterized. Cells carrying these mutations were unable to use glucose as a carbon source as measured in turbidimetric growth experiments. Tetrad analysis indicated that these mutations were not linked to each other; one of the mutations, that affecting phosphoglycerate kinase, was located on chromosome III.
摘要
分离出了携带隐性突变的酵母菌株，这些突变代表了四个不同的基因座，它们分别导致丙酮酸激酶、丙酮酸脱羧酶、3-磷酸甘油酸激酶和3-磷酸甘油酸变位酶出现缺陷，并对其进行了部分表征。在比浊生长实验中，携带这些突变的细胞无法将葡萄糖用作碳源。四分体分析表明，这些突变彼此不连锁；其中一个影响磷酸甘油酸激酶的突变位于第三条染色体上。
相似文献
1
Isolation and characterization of Saccharomyces cerevisiae glycolytic pathway mutants.酿酒酵母糖酵解途径突变体的分离与鉴定
J Bacteriol. 1977 May;130(2):746-9. doi: 10.1128/jb.130.2.746-749.1977.
2
Simultaneous overexpression of enzymes of the lower part of glycolysis can enhance the fermentative capacity of Saccharomyces cerevisiae.糖酵解下游部分酶的同时过表达可增强酿酒酵母的发酵能力。
Yeast. 2000 Oct;16(14):1325-34. doi: 10.1002/1097-0061(200010)16:14<1325::AID-YEA627>3.0.CO;2-E.
3
Regulation of phosphoenolpyruvate carboxykinase and pyruvate kinase in Saccharomyces cerevisiae grown in the presence of glycolytic and gluconeogenic carbon sources and the role of mitochondrial function on gluconeogenesis.在存在糖酵解和糖异生碳源的条件下生长的酿酒酵母中磷酸烯醇式丙酮酸羧激酶和丙酮酸激酶的调控以及线粒体功能在糖异生中的作用
Can J Microbiol. 1986 Dec;32(12):969-72. doi: 10.1139/m86-180.
4
The GCR1 requirement for yeast glycolytic gene expression is suppressed by dominant mutations in the SGC1 gene, which encodes a novel basic-helix-loop-helix protein.酵母糖酵解基因表达对GCR1的需求被SGC1基因中的显性突变所抑制，该基因编码一种新型的碱性螺旋-环-螺旋蛋白。
Mol Cell Biol. 1995 May;15(5):2646-53. doi: 10.1128/MCB.15.5.2646.
5
Human acylphosphatase cannot replace phosphoglycerate kinase in Saccharomyces cerevisiae.人酰基磷酸酶不能替代酿酒酵母中的磷酸甘油酸激酶。
Antonie Van Leeuwenhoek. 2001 Oct;80(1):11-7. doi: 10.1023/a:1012082312137.
6
Isolation and characterization of a Saccharomyces cerevisiae mutant deficient in pyruvate kinase activity.一株丙酮酸激酶活性缺陷型酿酒酵母突变体的分离与鉴定
J Bacteriol. 1977 Apr;130(1):232-41. doi: 10.1128/jb.130.1.232-241.1977.
7
Physiological effects of seven different blocks in glycolysis in Saccharomyces cerevisiae.酿酒酵母中糖酵解的七种不同阻断的生理效应。
J Bacteriol. 1979 Jul;139(1):152-60. doi: 10.1128/jb.139.1.152-160.1979.
8
Induction of pyruvate decarboxylase in glycolysis mutants of Saccharomyces cerevisiae correlates with the concentrations of three-carbon glycolytic metabolites.酿酒酵母糖酵解突变体中丙酮酸脱羧酶的诱导与三碳糖酵解代谢物的浓度相关。
Arch Microbiol. 1993;160(4):324-8. doi: 10.1007/BF00292085.
9
The glucose-6-phosphate-isomerase reaction is essential for normal glucose repression in Saccharomyces cerevisiae.葡萄糖-6-磷酸异构酶反应对于酿酒酵母中的正常葡萄糖阻遏至关重要。
Eur J Biochem. 1993 May 15;214(1):121-7. doi: 10.1111/j.1432-1033.1993.tb17903.x.
10
Saccharomyces cerevisiae aldolase mutants.酿酒酵母醛缩酶突变体
J Bacteriol. 1984 Oct;160(1):222-6. doi: 10.1128/jb.160.1.222-226.1984.
引用本文的文献
1
The Role of Transcription Factors at Antisense-Expressing Gene Pairs in Yeast.转录因子在酵母中反义表达基因对中的作用
Genome Biol Evol. 2016 Jun 27;8(6):1748-61. doi: 10.1093/gbe/evw104.
2
Isolation and characterization of a maltose transport mutant in the yeast Saccharomyces cerevisiae.酵母酿酒酵母麦芽糖运输突变体的分离与鉴定。
Curr Genet. 1983 Jun;7(3):195-9. doi: 10.1007/BF00434890.
3
Forward chemical genetics in yeast for discovery of chemical probes targeting metabolism.在酵母中进行正向化学遗传学研究，以发现针对代谢的化学探针。
Molecules. 2012 Nov 5;17(11):13098-115. doi: 10.3390/molecules171113098.
4
Evolutionary significance of metabolic network properties.代谢网络性质的进化意义。
J R Soc Interface. 2012 Jun 7;9(71):1168-76. doi: 10.1098/rsif.2011.0652. Epub 2011 Nov 30.
5
An engineered amino-terminal domain of yeast phosphoglycerate kinase with native-like structure.具有天然样结构的酵母磷酸甘油酸激酶工程化氨基末端结构域。
Protein Sci. 1997 Apr;6(4):882-91. doi: 10.1002/pro.5560060415.
6
The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates.解脂耶氏酵母的3-磷酸甘油酸激酶基因在糖异生底物上会去阻遏。
Curr Genet. 1996 Apr;29(5):446-56. doi: 10.1007/BF02221513.
7
Disruption of the 3' phosphoglycerate kinase (pgk) gene in Aspergillus nidulans.构巢曲霉中3'-磷酸甘油酸激酶（pgk）基因的破坏。
Curr Genet. 1993 Feb;23(2):123-8. doi: 10.1007/BF00352010.
8
Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis.酵母糖酵解中丙酮酸脱羧酶反应的遗传分析。
J Bacteriol. 1982 Sep;151(3):1146-52. doi: 10.1128/jb.151.3.1146-1152.1982.
9
Petite-negative mutants of Saccharomyces cerevisiae.酿酒酵母的小菌落阴性突变体。
Mol Gen Genet. 1981;181(3):409-10. doi: 10.1007/BF00425622.
10
Isolation of the centromere-linked CDC10 gene by complementation in yeast.通过酵母互补作用分离着丝粒连接的CDC10基因。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2173-7. doi: 10.1073/pnas.77.4.2173.
本文引用的文献
1
Biochemical Mutants in the Smut Fungus Ustilago Maydis.玉米黑粉菌中的生化突变体
Genetics. 1949 Sep;34(5):607-26. doi: 10.1093/genetics/34.5.607.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Use of snail digestive juice in isolation of yeast spore tetrads.蜗牛消化液在酵母孢子四分体分离中的应用。
J Bacteriol. 1959 Aug;78(2):292. doi: 10.1128/jb.78.2.292-292.1959.
4
Molecular weight and coenzyme content of pyruvate decarboxylase from brewer's yeast.啤酒酵母丙酮酸脱羧酶的分子量及辅酶含量
Biochim Biophys Acta. 1966 Mar 7;113(3):595-604. doi: 10.1016/s0926-6593(66)80017-6.
5
A kinetic study of glycolytic enzyme synthesis in yeast.酵母中糖酵解酶合成的动力学研究。
J Biol Chem. 1971 Jan 25;246(2):475-88.
6
Purification and crystallization of yeast pyruvate kinase.酵母丙酮酸激酶的纯化与结晶
Hoppe Seylers Z Physiol Chem. 1972 Mar;353(3):435-40. doi: 10.1515/bchm2.1972.353.1.435.
7
Carbohydrate metabolism in microorganisms.微生物中的碳水化合物代谢
Annu Rev Microbiol. 1969;23:539-78. doi: 10.1146/annurev.mi.23.100169.002543.
8
Isolation and characterization of Escherichia coli mutants defective in enzymes of glycolysis.糖酵解酶缺陷型大肠杆菌突变体的分离与鉴定。
Biochem Biophys Res Commun. 1974 Jan;56(1):127-33. doi: 10.1016/s0006-291x(74)80324-4.
9
A revised preparation of yeast (Saccharomyces cerevisiae) pyruvate kinase.酵母（酿酒酵母）丙酮酸激酶的改良制剂。
J Biol Chem. 1976 Jan 10;251(1):124-8.
10
Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure.丙酮酸脱羧酶III。蛋白质结合步骤中硫胺素焦磷酸的特异性限制，亚基结构。
Biochim Biophys Acta. 1975 Oct 20;405(2):492-9.
Zotero 插件