包被抗体的含蛋白A金黄色葡萄球菌：一种多功能且稳定的免疫试剂。

Antibody-coated protein A-bearing Staphylococcus aureus: a versatile and stable immune reagent.

作者信息

Natali P G, Pellegrino M A, Walker L, Ferrone S, Reisfeld R A

出版信息

J Immunol Methods. 1979;25(3):255-64. doi: 10.1016/0022-1759(79)90113-3.

Abstract

The human beta2-microglobulin antigen-antibody system was used as a model to illustrate the versatility of a microradioimmunoassay technique using protein A-bearing Staphylococcus aureus Cowan I strain (SACI) bacteria as a non-specific immunoadsorbent in place of a second antibody. Experimental conditions are described for a sensitive microassay which makes it possible to process large numbers of samples more rapidly and with minimum handling. Furthermore, SACI coated with specific antibodies by mixing with unfractionated antisera are a versatile reagent not only for radioimmunoassays but also for use as molecular probes to characterize cell surface antigens. Antibody-coated SACI could be lyophilized and proved extremely stable in storage thus providing a unique advantage for use in binding inhibition assays and as versatile reagent for clinical and investigative immunology.

摘要

人类β2-微球蛋白抗原-抗体系统被用作一个模型，以说明一种微放射免疫测定技术的多功能性。该技术使用携带蛋白A的金黄色葡萄球菌考恩I株（SACI）细菌作为非特异性免疫吸附剂来替代第二抗体。描述了一种灵敏的微量测定的实验条件，该条件使得能够更快速且以最少的操作处理大量样本。此外，通过与未分级抗血清混合而包被有特异性抗体的SACI不仅是放射免疫测定的多功能试剂，还可用作表征细胞表面抗原的分子探针。包被抗体的SACI可以冻干，并且在储存中被证明极其稳定，从而为用于结合抑制测定以及作为临床和研究免疫学的多功能试剂提供了独特优势。