采用不同生长培养基，运用Etest法检测淋病奈瑟菌分离株抗菌药敏性的评估
Evaluation of Etest for testing antimicrobial susceptibilities of Neisseria gonorrhoeae isolates with different growth media.
作者信息
Yeung K H, Ng L K, Dillon J A
机构信息
National Laboratory for Sexually Transmitted Diseases, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
出版信息
J Clin Microbiol. 1993 Nov;31(11):3053-5. doi: 10.1128/jcm.31.11.3053-3055.1993.
Abstract
The MICs for 101 isolates of Neisseria gonorrhoeae obtained by Etest (AB-Biodisk, Solna, Sweden) and the agar dilution method on GC medium base supplemented with 1% Kellog's defined supplement (GCMB) were compared. The overall percent agreement (within 1 log2 dilution) between methods was greater than 97.9. The Pearson's correlation coefficients for penicillin, tetracycline, erythromycin, and ceftriaxone for the two methods were 0.98, 0.97, 0.93, and 0.93 (P = 0.001), respectively, for comparisons on GCMB. The overall percent agreement was lower when hemoglobin-supplemented GCMB was used. Etest is an attractive alternative to the agar dilution method for gonococcal antimicrobial susceptibility testing and should be further analyzed in multicenter studies.
摘要
比较了通过Etest（AB-生物盘公司，瑞典索尔纳）和琼脂稀释法在添加1%凯洛格特定添加剂（GCMB）的淋病奈瑟菌培养基基础上对101株淋病奈瑟菌分离株测定的最低抑菌浓度（MIC）。两种方法之间的总体一致百分比（在1个对数2稀释范围内）大于97.9。在GCMB上进行比较时，两种方法针对青霉素、四环素、红霉素和头孢曲松的Pearson相关系数分别为0.98、0.97、0.93和0.93（P = 0.001）。当使用添加血红蛋白的GCMB时，总体一致百分比更低。对于淋球菌抗菌药物敏感性试验，Etest是琼脂稀释法的一种有吸引力的替代方法，应在多中心研究中进一步分析。
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