Mercer A R, Mobbs P G, Davenport A P, Evans P D
Cell Tissue Res. 1983;234(3):655-77. doi: 10.1007/BF00218658.
Fluorescence histochemistry with glyoxylic acid has been used in close conjunction with detailed anatomical studies (Mobbs 1982) to investigate the distribution of fluorogenic amines in the brain of the honeybee, Apis mellifera. In addition, the concentration and distribution of biogenic amines in the brain of the bee have been determined using highly sensitive radioenzymatic techniques and high performance liquid chromatography. The cerebral ganglia of the bee contain similar amounts of dopamine and serotonin, more dopamine than octopamine, and very low levels of noradrenaline. Slow fading green fluorescence, typical of catecholamines, was located throughout the cerebral ganglia, and was particularly intense in the central body and mushroom body neuropils. Results indicate that the fluorescence in the mushroom body neuropils is largely extrinsic in origin. Both dopamine and serotonin were detected in the calyces and alpha-lobe of the mushroom bodies, and in the antennal lobe. In the optic lobe, however, serotonin was found, but only low levels of dopamine were detected. Slow fading green fluorescence was replaced in the optic lobes by fluorescence which faded rapidly in the excitation light. The non-fluorogenic amine octopamine was found in the mushroom bodies and in the neuropils of the optic lobes, with the largest amounts of octopamine in the optic lobes associated with the neuropil of the medulla. The possibility that intrinsic neurones of the mushroom body neuropil are octopaminergic is discussed.
利用乙醛酸荧光组织化学技术，紧密结合详细的解剖学研究（莫布斯，1982年），对蜜蜂（西方蜜蜂）大脑中荧光胺的分布进行了研究。此外，还使用高灵敏度放射酶技术和高效液相色谱法测定了蜜蜂大脑中生物胺的浓度和分布。蜜蜂的脑神经节中多巴胺和血清素含量相近，多巴胺含量高于章鱼胺，去甲肾上腺素含量极低。典型的儿茶酚胺类物质呈现的缓慢消退的绿色荧光遍布整个脑神经节，在中央体和蘑菇体神经纤维网中尤为强烈。结果表明，蘑菇体神经纤维网中的荧光在很大程度上源于外部。在蘑菇体的花萼和α叶以及触角叶中均检测到了多巴胺和血清素。然而，在视叶中仅发现了血清素，检测到的多巴胺水平较低。视叶中缓慢消退的绿色荧光被在激发光下迅速消退的荧光所取代。在蘑菇体和视叶神经纤维网中发现了非荧光胺章鱼胺，视叶中与髓质神经纤维网相关的章鱼胺含量最高。文中讨论了蘑菇体神经纤维网的内在神经元为章鱼胺能神经元的可能性。
