人类Ia抗原的结构异质性和多态性分析。在DR1,1纯合子和DR3,W6杂合子B细胞系的Ia库中共同表达四种不同的分子亚群。
Analysis of the structural heterogeneity and polymorphism of human Ia antigens. Four distinct subsets of molecules are coexpressed in the Ia pool of both DR1,1 homozygous and DR3,W6 heterozygous B cell lines.
作者信息
Accolla R S
出版信息
J Exp Med. 1984 Feb 1;159(2):378-93. doi: 10.1084/jem.159.2.378.
Abstract
Four monoclonal antibodies reacting with distinct human Ia antigenic determinants have been used to demonstrate the coexpression of four distinct subsets, NG1, NG2, H40+-3/4+, and DC1 H40--3/4+, in the Ia pool of DR heterozygous or homozygous B cell lines. By two-dimensional peptide mapping the four subsets within the same Ia pool displayed structurally different beta as well as alpha subunits. The beta chain of the NG1 subset was shown to display considerable structural polymorphism when analyzed in two cell lines with distinct DR but similar DC phenotype, LG2 (DR1,1-DC1) and Raji (DR3, W6-DC1). In contrast, the beta chains of NG2, DC1 H40+-3/4+, and DC1 H40--3/4+ subsets of LG2 cells were shown to be very similar to their homologous Raji cell counterparts, thus indicating a relatively low structural polymorphism. Furthermore, the alpha chains of either one of the four subsets expressed in LG2 cells displayed very high structural similarities to the homologous counterparts in the Raji Ia pool, thus suggesting a relatively low polymorphism for the large Ia subunits described in this study. A striking feature deduced from this study was the selective subunit association of the distinct alpha-beta heterodimers.
摘要
四种与不同人类Ia抗原决定簇反应的单克隆抗体已被用于证明在DR杂合或纯合B细胞系的Ia库中，四种不同亚群NG1、NG2、H40 + -3/4 +和DC1 H40 - -3/4 +的共表达。通过二维肽图谱分析，同一Ia库中的四个亚群显示出结构不同的β亚基以及α亚基。当在具有不同DR但相似DC表型的两种细胞系LG2（DR1,1 - DC1）和Raji（DR3，W6 - DC1）中进行分析时，NG1亚群的β链显示出相当大的结构多态性。相比之下，LG2细胞的NG2、DC1 H40 + -3/4 +和DC1 H40 - -3/4 +亚群的β链与它们在Raji细胞中的同源对应物非常相似，因此表明结构多态性相对较低。此外，LG2细胞中表达的四个亚群中任何一个的α链与Raji Ia库中的同源对应物显示出非常高的结构相似性，因此表明本研究中描述的大Ia亚基的多态性相对较低。从这项研究中推断出的一个显著特征是不同α-β异二聚体的选择性亚基关联。
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