Klinkert J, Klein A
J Virol. 1978 Mar;25(3):730-7. doi: 10.1128/JVI.25.3.730-737.1978.
Ring-to-ring (early) replication of bacteriophage lambda DNA was blocked after heat inactivation of the P protein. Rolling circle (late) replication continued for several rounds at the rate reached when the temperature shift was carried out. The same differential effect was observed after inhibition of RNA or protein synthesis during the two different phases of replication. In contrast, inactivation of the O protein resulted in a fast stop of lambda DNA synthesis at early and late times after infection. The results were consistent with the following interpretations. (i) The lambda P gene product plays a role in the initiation of the ring-to-ring replication. (ii) Ring-to-ring replication continues parallel to rolling circle replication, possibly diminishing with time after infection. (iii) The O function is stable in and necessary for the structural integrity of an elongation complex. It is unstable in free form and probably released from such a replication complex after each round of replication at the ring-to-ring stage.
噬菌体λ DNA 的环对环（早期）复制在 P 蛋白热失活后被阻断。滚环（晚期）复制以温度转换时达到的速率持续进行了几轮。在复制的两个不同阶段抑制 RNA 或蛋白质合成后也观察到了相同的差异效应。相反，O 蛋白失活导致感染后早期和晚期λ DNA 合成迅速停止。结果与以下解释一致。（i）λ P 基因产物在环对环复制的起始中起作用。（ii）环对环复制与滚环复制并行持续，可能在感染后随时间减少。（iii）O 功能在延伸复合物的结构完整性中是稳定的且是必需的。它以游离形式不稳定，并且可能在环对环阶段的每一轮复制后从这样的复制复合物中释放出来。
