Sher G, Knutzen V K, Stratton C J, Montakhab M M, Allenson S G
Fertil Steril. 1984 Feb;41(2):260-4. doi: 10.1016/s0015-0282(16)47601-6.
Fourteen couples with long-standing infertility, associated with cervical mucus insufficiency, male subfertility, or unexplained infertility, participated in a therapeutic trial. The female partners, who were all ovulatory, were given human menopausal gonadotropin from day 2 of the menstrual cycle (controlled ovarian hyperstimulation). When plasma estradiol concentrations reached 1000 to 2000 pg/ml, human chorionic gonadotropin was given. Approximately 32 hours and again 70 hours thereafter, a masturbation specimen of the husband's sperm was capacitated in vitro and inseminated transcervically into the uterine cavity. Five women (35%) conceived following a single cycle of treatment. Four of the pregnancies are currently progressing normally; one ended in a spontaneous miscarriage in the early first trimester. The potential role of in vitro sperm capacitation and transcervical intrauterine insemination in the treatment of refractory infertility unrelated to female organic pelvic disease is discussed.
十四对患有长期不孕症的夫妇参与了一项治疗试验，这些不孕症与宫颈黏液分泌不足、男性生育力低下或不明原因的不孕有关。所有女性伴侣均有排卵，在月经周期的第2天给予人绝经期促性腺激素（控制性卵巢过度刺激）。当血浆雌二醇浓度达到1000至2000 pg/ml时，给予人绒毛膜促性腺激素。大约32小时后以及此后70小时再次采集丈夫的手淫精液标本，在体外进行精子获能处理，然后经宫颈将其注入子宫腔。五个女性（35%）在一个治疗周期后怀孕。目前有四个妊娠进展正常；一个在孕早期发生自然流产。本文讨论了体外精子获能和经宫颈子宫内授精在治疗与女性器质性盆腔疾病无关的难治性不孕症中的潜在作用。
