在无脊椎动物提取物中检测与花生四烯酸转化产物免疫相关的化合物。

Detection of compounds immunologically related to arachidonic acid transformation products in extracts of invertebrates.

作者信息

Levine L, Kobayashi T

出版信息

Prostaglandins Leukot Med. 1983 Dec;12(4):357-69. doi: 10.1016/0262-1746(83)90026-4.

Abstract

Organic extracts of Terpios zeteki, a sponge from the phylum Porifera, Aplysia californica and Helix aspersa, two species from the phylum Mollusca, and Arbacia, the sea urchin, from the phylum Echinodermata, contained compounds that appeared by radioimmunoassays to be HETE, PGE2, PGF2 alpha, and 6-keto-PGF1 alpha, the non-enzymatic hydrolytic product of PGI2. However, lack of coincidence of their retention times on HPLC with authentic oxygenation products of arachidonic acid suggested that most of the immunologically active compounds were derivatives of arachidonic acid metabolites or products derived from polyunsaturated fatty acids other than arachidonic acid.

摘要

多孔动物门的一种海绵——泽氏萜海绵、软体动物门的两种物种——加州海兔和欧洲花园蜗牛以及棘皮动物门的海胆——紫球海胆的有机提取物中含有一些化合物，通过放射免疫测定法显示这些化合物为HETE、PGE2、PGF2α以及6-酮-PGF1α（PGI2的非酶促水解产物）。然而，它们在高效液相色谱上的保留时间与花生四烯酸的真实氧化产物不一致，这表明大多数具有免疫活性的化合物是花生四烯酸代谢物的衍生物或源自花生四烯酸以外的多不饱和脂肪酸的产物。