Koga Y, Nishihara M, Morii H
Biochim Biophys Acta. 1984 Mar 27;793(1):86-94.
In order to understand the phosphatidylglycerol turnover mechanism, especially the differential turnover of diacylated and unacylated glycerol moieties of the lipid, products of phosphatidylglycerol metabolism were surveyed in vivo in Bacillus subtilis W23 and an alkalophile, Bacillus sp. strain A007. When cells of B. subtilis W23 labeled with radioactive glycerol were chased, lipoteichoic acid accumulated 90% of the radioactivity lost from the unacylated glycerol moiety of phosphatidylglycerol. Also, lipids other that phosphatidylglycerol, except diacylglycerol, and glycerol and glycerophosphate incorporated much less radioactivity. The [32P]phosphoryl group was also transferred from phosphatidylglycerol to lipoteichoic acid almost quantitatively in B. subtilis W23. A unique metabolism of phosphatidylglycerol was found in Bacillus sp. strain A007 which lacked phosphoglycolipid and lipoteichoic acid, that is, the turnover of phosphatidylglycerol of this organism was less extensive compared with that of B. subtilis W23, and both glycerol moieties of the lipid were metabolized at an identical rate. These results suggested that the major reaction involved in the turnover of phosphatidylglycerol was the transfer of glycerophosphate residue to lipoteichoic acid in a bacterium which possessed lipoteichoic acid and that several minor reactions also were involved in phosphatidylglycerol turnover.
为了了解磷脂酰甘油的周转机制，尤其是该脂质中二酰化和未酰化甘油部分的差异周转情况，我们在枯草芽孢杆菌W23和嗜碱芽孢杆菌A007菌株中对磷脂酰甘油代谢产物进行了体内研究。当用放射性甘油标记的枯草芽孢杆菌W23细胞进行追踪时，脂磷壁酸积累了磷脂酰甘油未酰化甘油部分损失的90%的放射性。此外，除二酰基甘油外的其他磷脂酰甘油以外的脂质、甘油和甘油磷酸掺入的放射性要少得多。在枯草芽孢杆菌W23中，[32P]磷酸基团也几乎定量地从磷脂酰甘油转移到脂磷壁酸。在缺乏磷酸糖脂和脂磷壁酸的嗜碱芽孢杆菌A007菌株中发现了一种独特的磷脂酰甘油代谢，即该生物体中磷脂酰甘油的周转与枯草芽孢杆菌W23相比不太广泛，并且该脂质的两个甘油部分以相同的速率代谢。这些结果表明，在具有脂磷壁酸的细菌中，磷脂酰甘油周转涉及的主要反应是甘油磷酸残基向脂磷壁酸的转移，并且磷脂酰甘油周转还涉及几个次要反应。
