使用碘甘醚对促甲状腺素释放激素进行碘化。
Iodination of thyroliberin by use of Iodogen.
作者信息
Butler S R, Lam R W, Fisher D A
出版信息
Clin Chem. 1984 Apr;30(4):547-8.
PMID:6423318
Abstract
In this method of labeling thyroliberin for radioimmunoassay, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril (Iodogen) is used as oxidant. By cation-exchange chromatography and in response to antibody, thyroliberin so labeled behaved similarly to that produced by the conventional Chloramine-T method. Major advantages of the Iodogen method are its reliability, simplicity, and higher yield of stable RIA label in its most convenient (diiodinated) form.
摘要
在这种用于放射免疫分析的促甲状腺素释放激素标记方法中，1,3,4,6-四氯-3α,6α-二苯基甘脲（碘代甘脲）用作氧化剂。通过阳离子交换色谱法并针对抗体，如此标记的促甲状腺素释放激素的行为与传统氯胺-T法产生的促甲状腺素释放激素相似。碘代甘脲法的主要优点是其可靠性、简便性以及以最方便的（二碘化）形式获得更高产量的稳定放射免疫分析标记物。
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