Wassom D L, Wakelin D, Brooks B O, Krco C J, David C S
Immunology. 1984 Apr;51(4):625-31.
H-2 congenic strains of mice were compared for their ability to expel T. spiralis infections from the small intestine and for their ability to limit the reproduction of adult female worms. B10.M mice (H-2f) expelled both primary and challenge infections more quickly than did the strains B10.Q(H-2q) and B10.BR(H-2k). During a primary infection, expulsion of worms from B10.M mice began before Day 9 post-infection and worm counts differed significantly (P less than .05 Student's t-test) from counts in B10.BR mice on each of Days 12 and 15.B10.Q mice expelled worms more rapidly than B10.BR but worm counts did not differ significantly until Day 15. Whereas B10.M mice responded most quickly to expel worms from the gut, B10.Q mice were most effective in limiting worm reproduction. Female worms harvested from B10.Q mice and cultured for 24 hr in vitro produced significantly fewer newborn larvae than did worms from B10.M or B10.BR mice. Worms from B10.M mice were less fecund than worms from B10.BR, but this difference was not apparent before Day 9 post-infection, and worms from B10.M were always more fecund than worms from B10.Q. Challenge infections 21 days following a priming dose of 200 T. spiralis muscle larvae were rejected very quickly. B10.M mice expelled 65% of their worms during the first 24 h. By Day 6 after challenge, B10.M mice had expelled 84% of their worms; B10.Q and B10.BR expelled 75% and 37% respectively. These results suggest that a rapid expulsion response may be expressed in many different strains of mice depending on how the mice are immunized and the size of the infecting dose. Fecundity of female worms 6 days following a challenge infection was reduced for all strains tested when compared to primary infection controls; however, worms from B10.Q mice were less fecund than worms from B10.M or B10.BR. Results of these experiments demonstrate that H-2 genes play an important role in controlling the immune response which expels worms from the gut and the response which limits worm reproduction. These H-2-controlled differences are expressed during both primary and challenge infections. As the present results conflict somewhat with results published elsewhere, we have proposed a new hypothesis to explain the data collected in our laboratories thus far. According to this hypothesis, the anti-adult response, the anti-fecundity response, and the rapid expulsion response are under independent genetic control and influenced by the interacting products of both H-2 and non-H-2 genes.
对H-2同源近交系小鼠排出小肠中旋毛虫感染的能力以及限制成年雌虫繁殖的能力进行了比较。B10.M小鼠（H-2f）排出初次感染和再次感染的速度比B10.Q（H-2q）和B10.BR（H-2k）品系更快。在初次感染期间，B10.M小鼠在感染后第9天之前就开始排出蠕虫，在第12天和第15天，其蠕虫计数与B10.BR小鼠的计数有显著差异（P<0.05，学生t检验）。B10.Q小鼠排出蠕虫的速度比B10.BR快，但直到第15天蠕虫计数才有显著差异。虽然B10.M小鼠对从肠道排出蠕虫的反应最快，但B10.Q小鼠在限制蠕虫繁殖方面最有效。从B10.Q小鼠收集的雌虫在体外培养24小时后产生的新生幼虫明显少于B10.M或B10.BR小鼠的蠕虫。B10.M小鼠的蠕虫繁殖力低于B10.BR小鼠，但这种差异在感染后第9天之前不明显，且B10.M小鼠的蠕虫繁殖力总是高于B10.Q小鼠。在接种200条旋毛虫肌幼虫的初免剂量21天后进行再次感染，蠕虫很快被排出。B10.M小鼠在最初24小时内排出了65%的蠕虫。到再次感染后第6天，B10.M小鼠排出了84%的蠕虫；B10.Q和B10.BR分别排出了75%和37%。这些结果表明，根据小鼠的免疫方式和感染剂量大小，许多不同品系的小鼠可能都会表现出快速的排虫反应。与初次感染对照组相比，所有受试品系在再次感染6天后雌虫的繁殖力均降低；然而，B10.Q小鼠的蠕虫繁殖力低于B10.M或B10.BR小鼠。这些实验结果表明，H-2基因在控制从肠道排出蠕虫的免疫反应以及限制蠕虫繁殖的反应中起重要作用。这些H-2控制的差异在初次感染和再次感染期间均有表现。由于目前的结果与其他地方发表的结果有些冲突，我们提出了一个新的假说来解释迄今为止在我们实验室收集的数据。根据这个假说，抗成虫反应、抗繁殖力反应和快速排虫反应受独立的基因控制，并受H-2和非H-2基因相互作用产物的影响。
