Bogardus C, Lillioja S, Stone K, Mott D
J Clin Invest. 1984 Apr;73(4):1185-90. doi: 10.1172/JCI111304.
We have studied the relationship between in vivo insulin-mediated glucose disposal rates, muscle glycogen content, and muscle glycogen synthase activity in 25 southwest American Indians with normal glucose tolerance and with varying degrees of glucose intolerance. Insulin-mediated glucose disposal (M) was measured by using the hyperinsulinemic euglycemic clamp technique at plasma insulin concentrations of 134 +/- 7 and 1709 +/- 72 microU/ml, with simultaneous indirect calorimetry to assess glucose oxidation and storage rates. Muscle glycogen content and glycogen synthase activity were measured in percutaneous muscle biopsy samples obtained from the vastus lateralis muscle before and after the euglycemic clamp procedure. The results showed that muscle glycogen synthase activity at the end of the euglycemic clamp was well correlated with insulin-mediated glucose storage rates at both low (r = 0.50, P less than 0.02) and high (r = 0.78, P less than 0.0001) insulin concentrations; and also correlated with M (r = 0.66, P less than 0.001 and r = 0.76, P less than 0.0001). Similar correlations were observed between the change in muscle glycogen synthase activity and glucose storage rates and M. The change in muscle glycogen synthase activity correlated with the change in muscle glycogen content (r = 0.46, P less than 0.03) measured before and after the insulin infusions. The change in muscle glycogen content did not correlate with glucose storage rates or M. The data suggest the possible importance of glycogen synthesis in muscle in determining in vivo insulin-mediated glucose disposal rates in man.
我们研究了25名糖耐量正常及糖耐量不同程度受损的美国西南部印第安人体内胰岛素介导的葡萄糖处置率、肌肉糖原含量和肌肉糖原合酶活性之间的关系。通过高胰岛素正常血糖钳夹技术，在血浆胰岛素浓度为134±7和1709±72微单位/毫升时测量胰岛素介导的葡萄糖处置（M），同时采用间接测热法评估葡萄糖氧化和储存率。在正常血糖钳夹程序前后，从股外侧肌获取经皮肌肉活检样本，测量肌肉糖原含量和糖原合酶活性。结果显示，正常血糖钳夹结束时的肌肉糖原合酶活性与低（r = 0.50，P<0.02）、高（r = 0.78，P<0.0001）胰岛素浓度下胰岛素介导的葡萄糖储存率均显著相关；也与M显著相关（r = 0.66，P<0.001和r = 0.76，P<0.0001）。肌肉糖原合酶活性变化与葡萄糖储存率及M之间也观察到类似的相关性。肌肉糖原合酶活性变化与胰岛素输注前后测量的肌肉糖原含量变化相关（r = 0.46，P<0.03）。肌肉糖原含量变化与葡萄糖储存率或M无关。这些数据表明，肌肉中糖原合成在决定人体胰岛素介导的葡萄糖处置率方面可能具有重要意义。
