Otokunefor T V, Galsworthy S B
J Leukoc Biol. 1984 Feb;35(2):143-56. doi: 10.1002/jlb.35.2.143.
An immunosuppressive agent (ISA) present in an aqueous extract from Listeria monocytogenes diminished the immune response in vivo to subsequently injected heterologous antigen. Intraperitoneal injection of ISA induced an inflammatory response and activation of the reticuloendothelial system, both of which coincided with the period of immune hyporesponsiveness. Mice treated with ISA exhibited increased accumulation of labelled antigen to phagocytic peritoneal cells but decreased delivery of labelled antigen to the spleen. Both delivery of antigen to spleen and the immune response could be improved by injecting either ISA or antigen or both intravenously, or by increasing the dose of antigen. The response of ISA-treated animals could also be improved by intraperitoneal injection of latex beads, colloidal carbon, or carrageenan shortly (60 min) before immunization. Spleen cells from ISA-treated mice adoptively transferred to irradiated syngeneic recipients were able to mount a normal immune response. These results suggest that ingestion of antigen by the enlarged population of phagocytes in peritoneal cavities of ISA-treated mice prevented antigen delivery to the spleen and was partly responsible for the observed immunosuppression.
单核细胞增生李斯特菌水提取物中存在的一种免疫抑制剂（ISA）在体内减弱了对随后注射的异源抗原的免疫反应。腹腔注射ISA会引发炎症反应并激活网状内皮系统，这两者都与免疫低反应期相吻合。用ISA处理的小鼠显示出标记抗原向吞噬性腹膜细胞的积累增加，但标记抗原向脾脏的递送减少。通过静脉注射ISA或抗原或两者，或通过增加抗原剂量，可以改善抗原向脾脏的递送和免疫反应。在免疫前不久（60分钟）腹腔注射乳胶珠、胶体碳或角叉菜胶也可以改善经ISA处理动物的反应。将经ISA处理小鼠的脾细胞过继转移到经照射的同基因受体中，这些脾细胞能够产生正常的免疫反应。这些结果表明，经ISA处理小鼠腹腔中大量吞噬细胞摄取抗原阻止了抗原向脾脏的递送，并且部分导致了观察到的免疫抑制。
