Zucker-Franklin D, Grusky G, Marcus A
Lab Invest. 1978 May;38(5):620-8.
Human peripheral blood leukocytes, when cultured in soft agar give rise to giant (100 to 500 micrometer.) "foam cells." Investigation of the origin and properties of the cells proved that they were derived from monocytes in that the cells adherent to glass after 24 hours in culture were phagocytic, elaborated lysozyme and bore receptors for complement and immunoglobulin. The increment in size was accounted for primarily by large inclusions which on histochemical and biochemical analyses were shown to consist predominantly fo neutral fat. Transformation to fat cells took place in the absence of mitosis. Fc receptors were retained but complement receptors were lost. These observations suggest a role for monocytes in the replacement of hematopoietic tissue by fat in certain hypoplastic states. The cultured monocytes may also serve to facilitate the study of fat synthesis and metabolism in vitro.
人外周血白细胞在软琼脂中培养时会产生巨大的（100至500微米）“泡沫细胞”。对这些细胞的起源和特性进行研究后发现，它们源自单核细胞，因为培养24小时后贴壁于玻璃的细胞具有吞噬作用，能产生溶菌酶，并带有补体和免疫球蛋白受体。细胞大小的增加主要是由于大量内含物，经组织化学和生化分析表明，这些内含物主要由中性脂肪组成。向脂肪细胞的转化在无有丝分裂的情况下发生。Fc受体得以保留，但补体受体丢失。这些观察结果表明，在某些发育不全状态下，单核细胞在脂肪替代造血组织中发挥作用。培养的单核细胞也可能有助于体外脂肪合成与代谢的研究。
