Penttila I A, Ey P L, Jenkin C R
Aust J Exp Biol Med Sci. 1983 Dec;61 ( Pt 6):617-27. doi: 10.1038/icb.1983.58.
Eosinophils and neutrophils, purified by density gradient centrifugation from the blood of infected mice resistant to reinfection, were tested for their ability to adhere to the different parasitic larval stages of the murine nematode parasite Nematospiroides dubius. Cells were tested for adherence to larvae which had been sensitised with immune mouse serum (IMS) or normal mouse serum (NMS) in the presence of CA2+ and Mg2+ ions. EDTA, or EGTA. Differences were observed in the degree of cell adherence to the different stages of the parasite. However, the adherence of the two cell types to any given stage of the parasite was similar. Adherence to the sheathed infective third-stage (L3) larvae, 96 h post-infective larvae and to adult worms depended to a large degree on conditions suitable for complement activation (viz. fresh serum and the presence of Ca2+ and Mg2+ ions). Complement was activated both via the alternative pathway by the parasite itself and via the classical pathway by parasite-bound antibodies. In these conditions, cell adherence probably occurred predominantly through the interaction of leucocyte third component of complement (C3) receptors with parasite-bound C3. In contrast, adherence of cells to exsheathed L3 and to the 48 h and 72 h post-infective larval stages appeared to involve antibody/Fc receptor as well as C3/C3 receptor interaction. The data indicate that N. dubius may undergo a series of antigenic changes during its life cycle and that antibodies capable of mediating granulocyte attachment are elicited predominantly against the early tissue developmental forms of the parasite.
通过密度梯度离心法从对再感染具有抗性的感染小鼠血液中纯化出嗜酸性粒细胞和中性粒细胞，检测它们黏附于鼠类线虫寄生虫杜氏旋毛虫不同寄生幼虫阶段的能力。在存在钙离子和镁离子、乙二胺四乙酸（EDTA）或乙二醇双四乙酸（EGTA）的情况下，检测细胞对用免疫小鼠血清（IMS）或正常小鼠血清（NMS）致敏的幼虫的黏附情况。观察到细胞对寄生虫不同阶段的黏附程度存在差异。然而，两种细胞类型对寄生虫任何给定阶段的黏附情况相似。对带鞘感染性第三期（L3）幼虫、感染后96小时的幼虫以及成虫的黏附在很大程度上取决于适合补体激活的条件（即新鲜血清以及钙离子和镁离子的存在）。补体可通过寄生虫自身的替代途径以及寄生虫结合抗体的经典途径被激活。在这些条件下，细胞黏附可能主要通过白细胞补体第三成分（C3）受体与寄生虫结合的C3之间的相互作用发生。相比之下，细胞对脱鞘L3以及感染后48小时和72小时幼虫阶段的黏附似乎涉及抗体/Fc受体以及C3/C3受体相互作用。数据表明，杜氏旋毛虫在其生命周期中可能会经历一系列抗原变化，并且能够介导粒细胞附着的抗体主要是针对寄生虫早期组织发育形式产生的。
