Pontisso P, Poon M C, Tiollais P, Brechot C
Br Med J (Clin Res Ed). 1984 May 26;288(6430):1563-6. doi: 10.1136/bmj.288.6430.1563.
The Southern transfer hybridisation technique was used to test mononuclear blood cells for hepatitis B virus DNA. Viral DNA sequences were detected in mononuclear cells of 10 out of 16 patients with hepatitis B virus infection and in none of 21 normal controls. Blood contamination was excluded by the absence of hepatitis B virus DNA in the corresponding serum samples in all cases. Free monomeric hepatitis B virus DNA was found in three patients positive for hepatitis Be antigen (HBeAg) and one positive for anti-HBe, and integrated hepatitis B virus DNA was present in four patients positive for anti-HBe. In two other patients the small size of the samples did not allow a distinction between free and integrated viral DNA. The state of the virus in the mononuclear cells seemed to correlate with the HBeAg or anti-HBe state, as has been noted in the liver. These results indicate that hepatitis B virus may infect mononuclear blood cells, thereby expanding the tissue specificity of this agent beyond the liver, as has been reported for pancreatic, kidney, and skin tissue. They also suggest that hepatitis B virus infection of mononuclear cells might be related to immunological abnormalities observed in carriers of the virus.
采用Southern印迹杂交技术检测单核血细胞中的乙肝病毒DNA。在16例乙肝病毒感染患者中，有10例患者的单核细胞检测到病毒DNA序列，而21例正常对照者均未检测到。所有病例的相应血清样本中均未检测到乙肝病毒DNA，排除了血液污染。在3例乙肝e抗原（HBeAg）阳性患者和1例抗-HBe阳性患者中发现了游离的单体乙肝病毒DNA，在4例抗-HBe阳性患者中存在整合的乙肝病毒DNA。在另外2例患者中，样本量过小无法区分游离病毒DNA和整合病毒DNA。单核细胞中的病毒状态似乎与HBeAg或抗-HBe状态相关，正如在肝脏中所观察到的那样。这些结果表明，乙肝病毒可能感染单核血细胞，从而使该病原体的组织特异性超出肝脏范围，正如之前报道的在胰腺、肾脏和皮肤组织中的情况。它们还提示，单核细胞的乙肝病毒感染可能与病毒携带者中观察到的免疫异常有关。
