Wylie J L, Iliffe E R, Wang L L, McClarty G
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Infect Immun. 1997 Apr;65(4):1527-30. doi: 10.1128/iai.65.4.1527-1530.1997.
The kdsA and kdsB genes from Chlamydia trachomatis encoding 3-deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase and CMP-KDO synthetase were identified by functional complementation of temperature-sensitive Salmonella typhimurium mutants, homology to known KDO-8-phosphate synthetase and CMP-KDO synthetase proteins, and in vitro enzyme activity. The kdsA gene was transcribed as part of a polycistronic mRNA with two downstream open reading frames (ORFs). One of these ORFs appeared to encode a membrane-anchored protein, while the second encoded a protein showing homology to the ATP-binding component of periplasmic binding protein-dependent ABC transporters. Transcription of kdsA and kdsB in C. trachomatis was evident within 4 h of initiation of the C. trachomatis infection process and continued throughout the chlamydial life cycle.
通过对温度敏感型鼠伤寒沙门氏菌突变体的功能互补、与已知的3-脱氧-D-甘露糖辛酮酸酯（KDO）-8-磷酸合成酶和CMP-KDO合成酶蛋白的同源性以及体外酶活性，鉴定了沙眼衣原体中编码KDO-8-磷酸合成酶和CMP-KDO合成酶的kdsA和kdsB基因。kdsA基因作为一个多顺反子mRNA的一部分进行转录，该mRNA带有两个下游开放阅读框（ORF）。其中一个ORF似乎编码一种膜锚定蛋白，而另一个编码的蛋白与周质结合蛋白依赖性ABC转运蛋白的ATP结合成分具有同源性。沙眼衣原体感染过程开始后4小时内，沙眼衣原体中kdsA和kdsB的转录明显可见，并在整个衣原体生命周期中持续存在。
