Matera L, Massaia M, Pegoraro L
J Clin Lab Immunol. 1984 Feb;13(2):85-8.
An active supernatant (Ly Con A) was prepared by stimulating the normal human lymph node lymphocytes with Concanavalin A (Con A). Peripheral blood lymphocytes (PBL) from 3 healthy subjects and from 8 patients with chronic lymphocytic leukaemia (CLL) were cultured in the presence of Con A and Ly Con A. The latter induced a significant DNA synthesis both in normal and CLL lymphocytes. A proliferative response was still present in CLL after T lymphocytes depletion. The Con A and Ly Con A treatment also induced morphological changes in CLL lymphocytes consistent with plasma cell differentiation. In 3 cases the appearance of cytoplasmic light chains was detected by immunofluorescence. These findings suggest that peripheral blood B lymphocytes from CLL patients can be stimulated to maturation when helper factor(s) released by mitogen activated lymph node lymphocytes are provided.
通过用刀豆球蛋白A（Con A）刺激正常人淋巴结淋巴细胞制备活性上清液（Ly Con A）。将来自3名健康受试者和8名慢性淋巴细胞白血病（CLL）患者的外周血淋巴细胞（PBL）在Con A和Ly Con A存在的情况下进行培养。后者在正常淋巴细胞和CLL淋巴细胞中均诱导了显著的DNA合成。T淋巴细胞耗竭后CLL中仍存在增殖反应。Con A和Ly Con A处理还诱导了CLL淋巴细胞与浆细胞分化一致的形态学变化。在3例中通过免疫荧光检测到细胞质轻链的出现。这些发现表明，当提供有丝分裂原激活的淋巴结淋巴细胞释放的辅助因子时，CLL患者的外周血B淋巴细胞可以被刺激成熟。
