Howarth B
Poult Sci. 1984 Apr;63(4):838-40. doi: 10.3382/ps.0630838.
The effect of various atmospheric CO2 concentrations on fertility of cock semen incubated in minimum essential medium (MEM) for 6 hr in vitro at 41 C was evaluated. Fertility following intravaginal insemination was 54, 87, and 78% for semen incubated in .03, 10, and 15% by volume CO2, respectively. No differences in hatchability of fertile eggs were observed between the semen treatments. Oxygen consumption values monitored at the beginning and end of the holding period were similar for semen incubated in 10% atmospheric CO2 but decreased for semen incubated in .03 and 15% CO2.
评估了在41℃下于最低必需培养基（MEM）中体外孵育6小时的不同大气二氧化碳浓度对公鸡精液受精能力的影响。通过阴道内授精后的受精率分别为：在体积分数为0.03%、10%和15%二氧化碳中孵育的精液，受精率分别为54%、87%和78%。在不同精液处理组之间，未观察到可育蛋孵化率的差异。在保存期开始和结束时监测的氧气消耗量，对于在10%大气二氧化碳中孵育的精液相似，但对于在0.03%和15%二氧化碳中孵育的精液则降低。
