Howarth B
Poult Sci. 1981 May;60(5):1075-8. doi: 10.3382/ps.0601075.
Cock spermatozoa were incubated for 6 and 24 hr at 41 C with minimum essential medium (MEM), tissue culture fluid (TCF) removed from tissue cultured cells and in the presence of tissue cultured cells (TCC). the TCC were obtained from whole 9-day-old chick embryos. Fertility following intravaginal artificial insemination was 75, 77, and 80% for semen incubated at 41 C for 6 hr in MEM, TCF, and TCC, respectively. Fertility of semen incubated for 24 hr was approximately 50% for all three treatment groups. The MEM alone was as beneficial as TCF or TCC in maintaining in vitro sperm survival. These observations provide a method for maintaining in vitro the fertilizing capacity (as evaluated by intravaginal insemination) of large numbers of cock spermatozoa under conditions of temperature comparable to those of the hen's oviduct.
将公鸡精子在41℃下，分别与最低限度基本培养基（MEM）、从组织培养细胞中取出的组织培养液（TCF）以及在有组织培养细胞（TCC）存在的条件下孵育6小时和24小时。TCC取自9日龄的完整鸡胚。经阴道人工授精后，在MEM、TCF和TCC中于41℃孵育6小时的精液的受精率分别为75%、77%和80%。在所有三个处理组中，孵育24小时的精液的受精率约为50%。单独的MEM在维持体外精子存活方面与TCF或TCC同样有益。这些观察结果提供了一种在与母鸡输卵管温度相当的条件下，体外维持大量公鸡精子受精能力（通过阴道内授精评估）的方法。
