Tsukamoto A, Weissman I L, Hunt S V
EMBO J. 1984 May;3(5):975-81. doi: 10.1002/j.1460-2075.1984.tb01916.x.
The frequency of normal rat peripheral B lymphocytes stained for surface immunoglobulin kappa allotypes a and b in (a X b) F1 heterozygotes was assessed by two-colour immunofluorescence on a fluorescence-activated cell sorter. The upper limit to the frequency of double- stainers was 8% among all kappa-positive cells, though it was not resolved how far cytophilic antibody accounted for these. Cells expressing each allotype singly were isolated and the extent of rearrangement of the genes encoding the joining-kappa segment on the expressed and non-expressed chromosome were independently assessed. The expressed allele was found to be virtually completely rearranged while the non-expressed allele showed approximately 45-60% rearrangement. The implications of this substantial non-productive rearrangement for models of allelic exclusion are discussed.
通过荧光激活细胞分选仪上的双色免疫荧光法，评估了正常大鼠外周B淋巴细胞表面免疫球蛋白κ同种异型a和b在（a×b）F1杂合子中的频率。在所有κ阳性细胞中，双染细胞的频率上限为8%，不过尚不清楚嗜细胞抗体在其中占多大比例。分别分离出单独表达每种同种异型的细胞，并独立评估了表达和未表达染色体上编码连接κ区段的基因的重排程度。结果发现，表达的等位基因几乎完全重排，而未表达的等位基因显示约45%-60%的重排。本文讨论了这种大量非生产性重排对等位基因排斥模型的影响。
