从患病羊毛中分离出的假单胞菌的特性分析。
Characterization of pseudomonads isolated from diseased fleece.
作者信息
London C J, Griffith I P
出版信息
Appl Environ Microbiol. 1984 May;47(5):993-7. doi: 10.1128/aem.47.5.993-997.1984.
A total of 59 Pseudomonas isolates was obtained from 11 samples of diseased fleece taken from live sheep. All but four of the isolates could be assigned to one of nine Pseudomonas species, of which P. aeruginosa, P. alcaligenes, P. mendocina , and P. putida were the most common. P. aeruginosa was found in four of the fleece samples and, when present, appeared to predominate. Although several of the isolates of P. aeruginosa lacked the ability to produce pyocyanin (and some produced neither pyocyanin nor fluorescein), nearly all produced several virulence factors. Of the other pseudomonads, many produced proteinase, esterase, and catalase, several were able to grow at 42 degrees C and reduce nitrate, and some also produced lipase and hemolysin and, like P. aeruginosa, might serve to initiate (or sustain) the dermatitis frequently associated with fleece rot in sheep.
从11只活羊身上采集的患病羊毛样本中总共获得了59株假单胞菌分离株。除4株外，所有分离株都可归为9种假单胞菌中的一种（铜绿假单胞菌、产碱假单胞菌、门多萨假单胞菌和恶臭假单胞菌最为常见）。在4份羊毛样本中发现了铜绿假单胞菌，一旦存在，似乎就占据主导地位。虽然几株铜绿假单胞菌分离株缺乏产生绿脓菌素的能力（有些既不产生绿脓菌素也不产生荧光素），但几乎所有菌株都产生了几种毒力因子。在其他假单胞菌中，许多菌株产生蛋白酶、酯酶和过氧化氢酶，有几种能够在42摄氏度下生长并还原硝酸盐，一些还产生脂肪酶和溶血素，并且像铜绿假单胞菌一样，可能引发（或维持）经常与绵羊腐蹄病相关的皮炎。
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