评估铜绿假单胞菌外毒素A和弹性蛋白酶作为急性肺部感染中的毒力因子。
Evaluation of Pseudomonas aeruginosa exotoxin A and elastase as virulence factors in acute lung infection.
作者信息
Blackwood L L, Stone R M, Iglewski B H, Pennington J E
出版信息
Infect Immun. 1983 Jan;39(1):198-201. doi: 10.1128/iai.39.1.198-201.1983.
Abstract
Acute Pseudomonas aeruginosa pneumonia was established in guinea pigs by intratracheal instillation of bacteria. Challenge strains included PAO-1, a strain known to produce exotoxin A, alkaline protease, and elastase, and several PAO-1 mutants deficient in either biologically active exotoxin A or elastase production. Survival, intrapulmonary killing of bacteria, and blood cultures were compared among the groups. Strains of P. aeruginosa deficient in active elastase production appeared to be less virulent than the parent strain and were more easily cleared from the lung. Opposite results were obtained for the exotoxin A-deficient mutants. These data suggest that elastase, but not exotoxin A, was an important virulence factor during acute pneumonia due to P. aeruginosa.
摘要
通过气管内注入细菌在豚鼠中建立急性铜绿假单胞菌肺炎。攻击菌株包括PAO-1，一种已知能产生外毒素A、碱性蛋白酶和弹性蛋白酶的菌株，以及几种缺乏生物活性外毒素A或弹性蛋白酶产生能力的PAO-1突变体。比较了各组之间的存活率、肺内细菌清除率和血培养结果。缺乏活性弹性蛋白酶产生能力的铜绿假单胞菌菌株似乎比亲本菌株的毒力更低，并且更容易从肺部清除。外毒素A缺陷突变体则得到相反的结果。这些数据表明，在铜绿假单胞菌引起的急性肺炎期间，弹性蛋白酶而非外毒素A是一种重要的毒力因子。
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本文引用的文献
1
In Vivo Studies with the Partially Purified Protease (Elastase) from Pseudomonas aeruginosa.用绿脓假单胞菌部分纯化的蛋白酶（弹性蛋白酶）进行体内研究。
Infect Immun. 1970 Nov;2(5):583-9. doi: 10.1128/iai.2.5.583-589.1970.
2
Elastin structure, biosynthesis, and relation to disease states.弹性蛋白的结构、生物合成及其与疾病状态的关系。
N Engl J Med. 1981 Mar 5;304(10):566-79. doi: 10.1056/NEJM198103053041004.
3
Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa.铜绿假单胞菌毒素A和弹性蛋白酶突变体小鼠的角膜感染
J Infect Dis. 1980 Oct;142(4):547-55. doi: 10.1093/infdis/142.4.547.
4
Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infections of rats.毒素A和弹性蛋白酶对铜绿假单胞菌在大鼠慢性肺部感染中致病性的作用。
Infect Immun. 1982 Jun;36(3):1223-8. doi: 10.1128/iai.36.3.1223-1228.1982.
5
Influence of mucoid coating on clearance of Pseudomonas aeruginosa from lungs.黏液样包被对铜绿假单胞菌从肺部清除的影响。
Infect Immun. 1981 May;32(2):443-8. doi: 10.1128/iai.32.2.443-448.1981.
6
The role of exotoxins in the pathogenesis of Pseudomonas aeruginosa infections.外毒素在铜绿假单胞菌感染发病机制中的作用。
J Infect Dis. 1980 Oct;142(4):626-30. doi: 10.1093/infdis/142.4.626.
7
Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase.产弹性蛋白酶改变的铜绿假单胞菌PAO突变体的分离与鉴定
J Bacteriol. 1980 Jun;142(3):836-42. doi: 10.1128/jb.142.3.836-842.1980.
8
Pseudomonas aeruginosa exotoxin A.铜绿假单胞菌外毒素A
N Engl J Med. 1980 Jun 12;302(24):1360-2. doi: 10.1056/NEJM198006123022410.
9
Isolation and characterization of a Pseudomonas aeruginosa mutant producing a nontoxic, immunologically crossreactive toxin A protein.一株产生无毒、免疫交叉反应性毒素A蛋白的铜绿假单胞菌突变体的分离与鉴定
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7199-203. doi: 10.1073/pnas.77.12.7199.
10
Extracellular toxins of Pseudomonas aeruginosa.铜绿假单胞菌的细胞外毒素
J Infect Dis. 1974 Nov;130 Suppl(0):S94-9. doi: 10.1093/infdis/130.supplement.s94.
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