Roberts B A, Glancy E M, Patterson D S
J Assoc Off Anal Chem. 1981 Jul;64(4):961-3.
A quantitative procedure widely used in European Economic Community (EEC) countries has been successfully scaled down to produce a rapid method for determination of aflatoxin B1 (and other aflatoxins) in animal feeds. Without modification, the method may be used for simultaneous ochratoxin A determination in simple feeds, but a slightly different extraction procedure is required for compound feeds. Validity of the method has been demonstrated by comparison with the full EEC procedure for aflatoxin B1 and the Nesheim method for ochratoxin A. Analyses may be completed within 2 h and there is a considerable savings in materials over the 2 reference methods. The procedure is also less hazardous because volumes of toxic extract are small, and the operator is exposed to minimum solvent vapor.
欧洲经济共同体（EEC）国家广泛使用的一种定量方法已成功缩小规模，从而产生了一种快速测定动物饲料中黄曲霉毒素B1（及其他黄曲霉毒素）的方法。无需修改，该方法可用于简单饲料中赭曲霉毒素A的同时测定，但复合饲料需要略有不同的提取程序。通过与黄曲霉毒素B1的完整EEC方法和赭曲霉毒素A的内斯海姆方法进行比较，证明了该方法的有效性。分析可在2小时内完成，与两种参考方法相比，材料成本大幅节省。该程序的危险性也较小，因为有毒提取物的量很小，操作人员接触的溶剂蒸汽最少。
