缬氨霉素、尼日利亚菌素和三氯卡班在控制大肠杆菌细胞质子动力中的应用。
The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells.
作者信息
Ahmed S, Booth I R
出版信息
Biochem J. 1983 Apr 15;212(1):105-12. doi: 10.1042/bj2120105.
Abstract
Valinomycin, nigericin and trichlorocarbanilide were assessed for their ability to control the protonmotive force in Escherichia coli cells. Valinomycin, at high K+ concentrations, was found to decrease the membrane potential delta phi and indirectly to decrease the pH gradient delta pH. Nigericin was found to have two modes of action. At low concentrations (0.05-2 microM) it carried out K+/H+ exchange and decreased delta pH. At higher concentrations (50 microM) it carried out a K+-dependent transfer of H+, decreasing both delta phi and delta pH. In EDTA-treated cells only the latter mode of action was evident, whereas in a mutant sensitive to deoxycholate both types of effect were observed. Trichlorocarbanilide is proposed as an alternative to nigericin for the specific control of delta pH, and it can be used in cells not treated with EDTA.
摘要
对缬氨霉素、尼日利亚菌素和三氯卡班进行了评估，以确定它们控制大肠杆菌细胞中质子动力的能力。发现在高钾离子浓度下，缬氨霉素会降低膜电位Δφ，并间接降低pH梯度ΔpH。发现尼日利亚菌素有两种作用模式。在低浓度（0.05 - 2微摩尔）时，它进行钾离子/氢离子交换并降低ΔpH。在较高浓度（50微摩尔）时，它进行依赖钾离子的氢离子转移，同时降低Δφ和ΔpH。在经乙二胺四乙酸（EDTA）处理的细胞中，仅后一种作用模式明显，而在对脱氧胆酸盐敏感的突变体中，两种类型的效应均被观察到。三氯卡班被提议作为尼日利亚菌素的替代品，用于特异性控制ΔpH，并且它可用于未用EDTA处理的细胞。
相似文献
1
The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells.缬氨霉素、尼日利亚菌素和三氯卡班在控制大肠杆菌细胞质子动力中的应用。
Biochem J. 1983 Apr 15;212(1):105-12. doi: 10.1042/bj2120105.
2
Energetically distinct early and late stages of HlyB/HlyD-dependent secretion across both Escherichia coli membranes.通过大肠杆菌的两层膜进行的HlyB/HlyD依赖性分泌在能量上不同的早期和晚期阶段。
EMBO J. 1991 Nov;10(11):3263-72. doi: 10.1002/j.1460-2075.1991.tb04890.x.
3
The proton electrochemical gradient in Escherichia coli cells.大肠杆菌细胞中的质子电化学梯度。
Eur J Biochem. 1976 Apr 1;63(2):533-41. doi: 10.1111/j.1432-1033.1976.tb10257.x.
4
ATP synthesis driven by protonmotive force imposed across Escherichia coli cell membranes.由跨大肠杆菌细胞膜施加的质子动力驱动的ATP合成。
FEBS Lett. 1975 Oct 1;57(3):290-3. doi: 10.1016/0014-5793(75)80319-x.
5
[The effect of valinomycin and nigericin on the efficacy of bacteriophage infection of staphylococcal cells].[缬氨霉素和尼日利亚菌素对葡萄球菌细胞噬菌体感染效力的影响]
Zh Mikrobiol Epidemiol Immunobiol. 1989 Feb(2):17-20.
6
The relationship between the electrochemical proton gradient and active transport in Escherichia coli membrane vesicles.大肠杆菌膜囊泡中电化学质子梯度与主动运输之间的关系。
Biochemistry. 1977 Mar 8;16(5):854-9. doi: 10.1021/bi00624a007.
7
The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles.大肠杆菌膜泡中质子的电化学梯度及其与主动运输的关系。
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1892-6. doi: 10.1073/pnas.73.6.1892.
8
Membrane potential in a potassium transport-negative mutant of Escherichia coli K-12. The distribution of rubidium in the presence of valinomycin indicates a higher potential than that of the tetraphenylphosphonium cation.大肠杆菌K-12钾转运阴性突变体中的膜电位。缬氨霉素存在下铷的分布表明其电位高于四苯基鏻阳离子的电位。
Biochim Biophys Acta. 1982 Sep 15;681(3):474-83. doi: 10.1016/0005-2728(82)90190-6.
9
Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 2. Effect of imposed delata psi, delta pH, and Delta mu H+.大肠杆菌膜囊泡中乳糖转运机制。2. 施加的膜电位差、pH 梯度和质子电化学势差的影响
Biochemistry. 1979 Aug 21;18(17):3697-704. doi: 10.1021/bi00584a010.
10
The proteoliposomal steady state. Effect of size, capacitance and membrane permeability on cytochrome-oxidase-induced ion gradients.蛋白脂质体稳态。大小、电容和膜通透性对细胞色素氧化酶诱导的离子梯度的影响。
Biochem J. 1990 Aug 15;270(1):109-18. doi: 10.1042/bj2700109.
引用本文的文献
1
Medical and Nonmedical Applications of Synthetic Transmembrane Anion Transporters.合成跨膜阴离子转运体的医学与非医学应用
Chem Rev. 2025 Sep 10;125(17):8370-8425. doi: 10.1021/acs.chemrev.5c00129. Epub 2025 Jul 7.
2
The ion channels of endomembranes.内质网膜的离子通道。
Physiol Rev. 2024 Jul 1;104(3):1335-1385. doi: 10.1152/physrev.00025.2023. Epub 2024 Mar 7.
3
Brevicidine acts as an effective sensitizer of outer membrane-impermeable conventional antibiotics for treatment.短杆菌肽可作为一种有效的增敏剂，用于增强外膜不通透性的传统抗生素的治疗效果。
Front Microbiol. 2023 Dec 15;14:1304198. doi: 10.3389/fmicb.2023.1304198. eCollection 2023.
4
Crucial Role for Lipoteichoic Acid Assembly in the Metabolic Versatility and Antibiotic Resistance of Staphylococcus aureus.脂磷壁酸装配在金黄色葡萄球菌代谢多样性和抗生素耐药性中的关键作用。
Infect Immun. 2023 Jul 18;91(7):e0055022. doi: 10.1128/iai.00550-22. Epub 2023 Jun 22.
5
Elucidating the Mechanism of Action of the Gram-Negative-Pathogen-Selective Cyclic Antimicrobial Lipopeptide Brevicidine.阐明革兰氏阴性病原体选择性环状抗菌脂肽布雷维菌素的作用机制。
Antimicrob Agents Chemother. 2023 May 17;67(5):e0001023. doi: 10.1128/aac.00010-23. Epub 2023 Mar 13.
6
Approach to nigericin derivatives and their therapeutic potential.尼日利亚菌素衍生物及其治疗潜力的研究方法。
RSC Adv. 2020 Nov 26;10(70):43085-43091. doi: 10.1039/d0ra05137c. eCollection 2020 Nov 23.
7
The potential convergence of NLRP3 inflammasome, potassium, and dopamine mechanisms in Parkinson's disease.NLRP3炎性小体、钾离子和多巴胺机制在帕金森病中的潜在关联。
NPJ Parkinsons Dis. 2022 Mar 24;8(1):32. doi: 10.1038/s41531-022-00293-z.
8
Active Efflux Leads to Heterogeneous Dissipation of Proton Motive Force by Protonophores in Bacteria.主动外排导致细菌中质子载体对质子动力的异质性耗散。
mBio. 2021 Aug 31;12(4):e0067621. doi: 10.1128/mBio.00676-21. Epub 2021 Jul 13.
9
BrevicidineB, a New Member of the Brevicidine Family, Displays an Extended Target Specificity.短杆菌肽B，短杆菌肽家族的新成员，具有更广泛的靶点特异性。
Front Microbiol. 2021 Jun 9;12:693117. doi: 10.3389/fmicb.2021.693117. eCollection 2021.
10
Use of a Fluorescence-Based Assay To Measure Escherichia coli Membrane Potential Changes in High Throughput.使用基于荧光的检测方法高通量测量大肠杆菌膜电位变化。
Antimicrob Agents Chemother. 2020 Aug 20;64(9). doi: 10.1128/AAC.00910-20.
本文引用的文献
1
Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism.通过化学渗透机制将磷酸化与电子及氢转移相偶联。
Nature. 1961 Jul 8;191:144-8. doi: 10.1038/191144a0.
2
The role of potassium transport in the generation of a pH gradient in Escherichia coli.钾离子转运在大肠杆菌pH梯度形成中的作用
Biochem J. 1981 Sep 15;198(3):691-8. doi: 10.1042/bj1980691.
3
Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion.Procoat是M13外壳蛋白的前体，其插入膜中需要电化学势。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4669-73. doi: 10.1073/pnas.77.8.4669.
4
5
Cation/proton antiport systems in Escherichia coli. Properties of the potassium/proton antiporter.大肠杆菌中的阳离子/质子反向转运系统。钾离子/质子反向转运体的特性。
J Biol Chem. 1980 Jan 10;255(1):39-44.
6
Membrane potential in a potassium transport-negative mutant of Escherichia coli K-12. The distribution of rubidium in the presence of valinomycin indicates a higher potential than that of the tetraphenylphosphonium cation.大肠杆菌K-12钾转运阴性突变体中的膜电位。缬氨霉素存在下铷的分布表明其电位高于四苯基鏻阳离子的电位。
Biochim Biophys Acta. 1982 Sep 15;681(3):474-83. doi: 10.1016/0005-2728(82)90190-6.
7
Proton chemical potential, proton electrical potential and bacterial motility.质子化学势、质子电势与细菌运动性
J Mol Biol. 1980 Apr 15;138(3):599-614. doi: 10.1016/s0022-2836(80)80019-2.
8
The effects of partial and selective reduction in the components of the proton-motive force on lactose uptake in Escherichia coli.质子动力势各组分的部分和选择性降低对大肠杆菌乳糖摄取的影响。
Biochem J. 1981 Dec 15;200(3):583-9. doi: 10.1042/bj2000583.
9
Quantitative measurements of the proton-motive force and its relation to steady state lactose accumulation in Escherichia coli.大肠杆菌中质子动力的定量测量及其与乳糖稳态积累的关系。
Biochem J. 1981 Dec 15;200(3):573-81. doi: 10.1042/bj2000573.
10
The sodium/proton antiporter is part of the pH homeostasis mechanism in Escherichia coli.钠/质子反向转运蛋白是大肠杆菌pH稳态机制的一部分。
J Biol Chem. 1982 Apr 10;257(7):3687-91.
Zotero 插件