细菌的感觉电生理学：枯草芽孢杆菌中膜电位与运动性及趋化性的关系。
Sensory electrophysiology of bacteria: relationship of the membrane potential to motility and chemotaxis in Bacillus subtilis.
作者信息
Miller J B, Koshland D E
出版信息
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4752-6. doi: 10.1073/pnas.74.11.4752.
Abstract
The relationship of membrane potential to motility and chemotaxis of Bacillus subtilis has been tested by using the fluorescence of a cyanine dye as a probe of the potential. The dye fluorescence was found to be an indicator of membrane potential by correlation with triphenylmethylphosphonium ion distribution and with changes due to anaerobicity and ionophore addition. When the potential was sufficient for motility and constant over time, it was found that the absolute level of the potential did not affect the swimming behavior of the bacteria. Transient alteration of the membrane potential did, however, lead to changes in swimming behavior. Attractants were found to alter the swimming behavior of the bacteria without altering the membrane potential. Thus, change of the overall membrane potential of a normal B. subtilis is not required for chemotaxis, but such a change is sensed by the bacteria just as changing levels of attractants and repellents are sensed.
摘要
通过使用花青染料的荧光作为电位探针，测试了枯草芽孢杆菌的膜电位与运动性和趋化性之间的关系。通过与三苯甲基鏻离子分布以及厌氧和添加离子载体引起的变化相关联，发现染料荧光是膜电位的指标。当电位足以支持运动且随时间保持恒定时，发现电位的绝对水平并不影响细菌的游动行为。然而，膜电位的瞬时改变确实会导致游动行为的变化。发现引诱剂会改变细菌的游动行为，而不会改变膜电位。因此，正常枯草芽孢杆菌的整体膜电位变化对于趋化性不是必需的，但细菌能感知这种变化，就如同能感知引诱剂和驱避剂水平的变化一样。
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