针对小鼠免疫球蛋白同种型的单克隆抗体。
Monoclonal antibodies to murine immunoglobulin isotypes.
作者信息
Weiss S, Lehmann K, Cohn M
出版信息
Hybridoma. 1983;2(1):49-54. doi: 10.1089/hyb.1983.2.49.
PMID:6432679
Abstract
We have obtained cell lines producing monoclonal antibodies against mu, alpha, lambda, and lambda 1 by fusing the mouse myeloma P3X63Ag8.653 with spleen cells from Lewis rats immunized with several lambda 1 bearing myeloma proteins. The anti-lambda 1 antibody is particularly useful. It was shown to be suitable for radioimmunoassays, for ELISAs when coupled to alkaline phosphatase, and for indirect fluorescence staining of the membrane as well as the cytoplasm.
摘要
我们通过将小鼠骨髓瘤P3X63Ag8.653与用几种携带λ1的骨髓瘤蛋白免疫的Lewis大鼠的脾细胞融合，获得了产生抗μ、α、λ和λ1单克隆抗体的细胞系。抗λ1抗体特别有用。已证明它适用于放射免疫测定、与碱性磷酸酶偶联时适用于酶联免疫吸附测定（ELISA），以及适用于膜和细胞质的间接荧光染色。
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