Epstein R, Lehmann K, Cohn M, Buckler C, Rowe W, Davisson M
Immunogenetics. 1984;19(6):527-37. doi: 10.1007/BF00403444.
Evidence is presented here for a close linkage between Akv-2, an ecotropic provirus found uniquely on chromosome 16 of AKR/N mice, and the immunoglobulin lambda 1 light chain locus, Igl-1. No recombinants between the Igl-1 locus and Akv-2 were found by Southern blot analysis of DNA obtained from progeny of the backcross of (AKR/N X SJL/J)F1 to SJL/J, indicating that these genes map within 5.9 cM of each other. A probe specific for the flanking sequence of Akv-2 was used to detect the provirus, while one specific for the Igl-1 constant region was used to determine which allele of the structural gene was expressed in the backcross mice. The constant region of Igl-1 differs between AKR/N and SJL/J with respect to a site for the restriction endonuclease KpnI. This backcross was also used to seek recombinants between the regulatory, Igl-1r, and structural, Igl-1, loci of the immunoglobulin light chain locus, since the existence of such recombinants would prove that these loci are distinct. Since only parental types were recovered in the offspring, the structural and regulatory loci are no more than 2.3 cM apart, and the implications of this finding are discussed.
本文提供的证据表明，Akv-2（一种仅在AKR/N小鼠16号染色体上发现的亲嗜性前病毒）与免疫球蛋白λ1轻链基因座Igl-1之间存在紧密连锁。通过对（AKR/N×SJL/J）F1与SJL/J回交后代的DNA进行Southern印迹分析，未发现Igl-1基因座与Akv-2之间的重组体，这表明这些基因彼此间的图谱距离在5.9厘摩以内。使用针对Akv-2侧翼序列的探针来检测前病毒，同时使用针对Igl-1恒定区的探针来确定结构基因的哪个等位基因在回交小鼠中表达。AKR/N和SJL/J的Igl-1恒定区在限制性内切酶KpnI的切割位点上存在差异。该回交还用于寻找免疫球蛋白轻链基因座的调节基因座Igl-1r和结构基因座Igl-1之间的重组体，因为此类重组体的存在将证明这些基因座是不同的。由于在后代中仅获得亲本类型，结构基因座和调节基因座的距离不超过2.3厘摩，并对这一发现的意义进行了讨论。
