在一株对硼雷素耐药的中国仓鼠卵巢细胞系中苏氨酰-tRNA合成酶水平升高。
Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line.
作者信息
Gantt J S, Bennett C A, Arfin S M
出版信息
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5367-70. doi: 10.1073/pnas.78.9.5367.
Abstract
The growth of Chinese hamster ovary cells in medium containing reduced concentrations of threonine is inhibited by borrelidin, a macrolide antibiotic. Borrelidin-resistant clones have been isolated after ethyl methanesulfonate mutagenesis. One clone, 1C-1, has a 3-fold increased level of threonyl-tRNA synthetase [L-threonine:tRNAThr ligase (AMP-forming), EC 6.1.1.3] as determined by both activity measurements and antiserum titrations. The levels of four other aminoacyl-tRNA synthetases and of tRNAThr are the same in strain 1C-1 and in the wild-type parent. The phenotype of increased threonyl-tRNA synthetase activity is recessive to wild type in cell hybrids.
摘要
在含有低浓度苏氨酸的培养基中，中国仓鼠卵巢细胞的生长会受到大环内酯类抗生素博来霉素的抑制。经甲磺酸乙酯诱变后，已分离出博来霉素抗性克隆。通过活性测定和抗血清滴定确定，其中一个克隆1C-1的苏氨酰-tRNA合成酶[L-苏氨酸：tRNAThr连接酶（形成AMP），EC 6.1.1.3]水平增加了3倍。1C-1菌株和野生型亲本中其他四种氨酰-tRNA合成酶以及tRNAThr的水平相同。在细胞杂交体中，苏氨酰-tRNA合成酶活性增加的表型相对于野生型是隐性的。
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