Hänsch G M, Seitz M, Martinotti G, Betz M, Rauterberg E W, Gemsa D
J Immunol. 1984 Oct;133(4):2145-50.
Rat peritoneal macrophages released arachidonic acid, prostaglandin E2, and thromboxane B2 when treated with normal rabbit or C6-deficient rabbit complement in vitro. Normal rabbit complement, however, was more efficient, which indicates that late complement components, in addition to the known effects of C3a and C3b, were responsible for an enhanced arachidonic acid turnover. Indeed, in the absence of the C3 cleavage products, the purified late complement components C5b6, C7, C8, and C9 stimulated the arachidonic acid, as well as the prostaglandin E2 and thromboxane B2 release. Incubation of C5b6, C7, C8, and C9 for 1 hr at 37 degrees C before addition to the macrophages abolished the stimulatory activity, being in complete agreement with the fact that a fluid phase-formed complex of C5-9 loses its membrane-binding capacity. Although the mechanism by which C5b-9-membrane interaction affects the arachidonic acid metabolism remains unclear, the data describe a new function of the late complement components.
当用正常兔或C6缺陷兔补体在体外处理时，大鼠腹膜巨噬细胞会释放花生四烯酸、前列腺素E2和血栓素B2。然而，正常兔补体更有效，这表明除了已知的C3a和C3b的作用外，补体晚期成分也导致花生四烯酸周转率增加。事实上，在没有C3裂解产物的情况下，纯化的补体晚期成分C5b6、C7、C8和C9会刺激花生四烯酸以及前列腺素E2和血栓素B2的释放。在加入巨噬细胞之前，将C5b6、C7、C8和C9在37℃孵育1小时会消除刺激活性，这与C5 - 9在液相形成的复合物失去其膜结合能力的事实完全一致。虽然C5b - 9与膜相互作用影响花生四烯酸代谢的机制仍不清楚，但这些数据描述了补体晚期成分的一种新功能。
