Goyns M H, Young B D, van Kessel A G, de Klein A, Grosveld G, Bartram C R, Bootsma D
Leuk Res. 1984;8(4):547-53. doi: 10.1016/0145-2126(84)90003-1.
The lambda light chain immunoglobulin constant region (C lambda) locus was mapped on human chromosome 22. A DNA probe containing part of the C lambda locus was isolated from a human chromosome 22 genomic library, and a series of rodent X human somatic cell hybrids (each of which contained different translocated parts of chromosome 22) were constructed and characterized. The hybridization of the C lambda probe to DNA from these cell hybrids was then studied by Southern blot analysis. The results demonstrates that the C lambda locus is situated very close to the translocation breakpoint on human chromosome 22 which is characteristic of chronic myeloid leukaemia, and at least part if not at all of the locus is situated on the Philadelphia chromosome.
λ轻链免疫球蛋白恒定区（Cλ）基因座被定位在人类22号染色体上。从人类22号染色体基因组文库中分离出一个包含部分Cλ基因座的DNA探针，并构建和鉴定了一系列啮齿动物×人类体细胞杂种（每个杂种包含22号染色体的不同易位部分）。然后通过Southern印迹分析研究Cλ探针与这些细胞杂种DNA的杂交情况。结果表明，Cλ基因座非常靠近人类22号染色体上慢性髓性白血病所特有的易位断点，并且该基因座至少部分（如果不是全部）位于费城染色体上。
