McBride O W, Hieter P A, Hollis G F, Swan D, Otey M C, Leder P
J Exp Med. 1982 May 1;155(5):1480-90. doi: 10.1084/jem.155.5.1480.
The chromosomal location of human constant region light chain immunoglobulin (Ig) genes has been determined by analyzing a group of human fibroblast/rodent somatic cell hybrids with nucleic acid probes prepared from cloned human kappa and lambda constant region genes. Human chromosomes in each cell line were identified by isoenzyme analysis. The DNA from hybrid cells was digested with restriction endonucleases, size fractionated by gel electrophoresis, transferred to nitrocellulose or DBM paper, and hybridized with (32)P-labeled nucleic acid probes. The C(kappa) gene was assigned to human chromosome 2 and the C(lambda) genes to chromosome 22, based upon analysis of these hybrid cell lines, and these assignments were confirmed by analysis of subclones. A group of previously unassigned loci can be mapped to chromosome 2 by virtue of their close linkage to C(kappa). The lambda and kappa light chain and heavy chain Ig genes have now been assigned to all three human chromosomes that are involved in translocations with chromosome 8 in human B cell neoplasms. These techniques and probes provide a means to study the detailed arrangement of human Ig genes and their pseudogenes.
通过用从克隆的人κ和λ恒定区基因制备的核酸探针分析一组人成纤维细胞/啮齿动物体细胞杂种，已确定了人恒定区轻链免疫球蛋白（Ig）基因的染色体定位。通过同工酶分析鉴定每个细胞系中的人染色体。用限制性内切酶消化杂种细胞的DNA，通过凝胶电泳进行大小分级，转移到硝酸纤维素或DBM纸上，并用（32）P标记的核酸探针进行杂交。基于对这些杂种细胞系的分析，将C（κ）基因定位于人第2号染色体，将C（λ）基因定位于第22号染色体，并且通过对亚克隆的分析证实了这些定位。一组先前未定位的基因座由于与C（κ）紧密连锁而可定位于第2号染色体。现在已将λ和κ轻链以及重链Ig基因定位于人类B细胞肿瘤中与第8号染色体发生易位的所有三条人类染色体上。这些技术和探针提供了一种研究人Ig基因及其假基因详细排列的方法。
