Thaete L G, Crouch R K, Buse M G, Spicer S S
Diabetologia. 1985 Sep;28(9):677-82. doi: 10.1007/BF00291975.
Copper-zinc superoxide dismutase is known to protect pancreatic beta-cell function from the damage of alloxan. A morphological investigation of copper-zinc superoxide dismutase prophylaxis against alloxan was undertaken in rats to investigate the mechanism of this protective action. Exogenous copper-zinc superoxide dismutase reproducibly protected the morphological features of pancreatic beta cells against damage by alloxan as determined by light microscopic immunostaining for insulin and by ultrastructural examination. By the same criteria, alpha and delta cells appeared unaffected by administration of a combination of alloxan and copper-zinc superoxide dismutase or either agent alone. Autoradiography after injection of 125I labelled copper-zinc superoxide dismutase into normal rats showed no evidence that the enzyme enters viable islet cells, suggesting an extracellular site of protection against alloxan.
已知铜锌超氧化物歧化酶可保护胰腺β细胞功能免受四氧嘧啶的损害。为研究这种保护作用的机制，对大鼠进行了铜锌超氧化物歧化酶预防四氧嘧啶损伤的形态学研究。通过胰岛素的光镜免疫染色和超微结构检查确定，外源性铜锌超氧化物歧化酶可重复性地保护胰腺β细胞的形态特征免受四氧嘧啶的损伤。根据相同标准，α细胞和δ细胞在注射四氧嘧啶和铜锌超氧化物歧化酶的组合或单独使用任何一种试剂后似乎未受影响。向正常大鼠注射125I标记的铜锌超氧化物歧化酶后的放射自显影显示，没有证据表明该酶进入活的胰岛细胞，这表明存在针对四氧嘧啶的细胞外保护位点。
