Biberfeld G, Arneborn P, Forsgren M, von Stedingk L V, Blomqvist S
Yale J Biol Med. 1983 Sep-Dec;56(5-6):639-42.
The ability of heat-killed Mycoplasma pneumoniae (MP) organisms to induce polyclonal antibody production in cultures of blood lymphocytes of healthy subjects was studied. MP induced both IgM and IgG production, with a predominance of IgM. Supernatants of MP-stimulated lymphocyte cultures were tested by an enzyme-linked immunosorbent assay for antibodies to measles, rubella, and herpes simplex virus. MP as well as pokeweed mitogen induced production of viral antibodies of IgG class in lymphocytes of donors who had serum antibodies to the corresponding viral antigens. The MP-induced non-specific antibody response was T-cell-dependent. Lymphocytes from four patients with MP pneumonia, collected nine to 13 days after onset of illness, were tested for in vitro Ig production in the absence of MP. These lymphocytes spontaneously produced increased amounts of IgM and/or IgG. Lymphocytes from three of these four patients spontaneously produced viral IgG antibodies to measles and/or varicella antigens, indicating that MP had induced non-specific activation of memory B cells in vivo. Spontaneous viral antibody production was not found in lymphocyte cultures of healthy donors. The non-specific activation of blood B cells in vitro is probably induced by non-specific helper factors from MP-activated T cells. It is possible that in vivo MP also may have a direct activating effect on B cells.
研究了热灭活肺炎支原体（MP）诱导健康受试者血液淋巴细胞培养物中多克隆抗体产生的能力。MP诱导IgM和IgG产生，以IgM为主。通过酶联免疫吸附测定法检测MP刺激的淋巴细胞培养物上清液中针对麻疹、风疹和单纯疱疹病毒的抗体。MP以及商陆有丝分裂原在对相应病毒抗原有血清抗体的供体淋巴细胞中诱导产生IgG类病毒抗体。MP诱导的非特异性抗体反应依赖于T细胞。在疾病发作后9至13天收集的4例MP肺炎患者的淋巴细胞，在无MP的情况下进行体外Ig产生检测。这些淋巴细胞自发产生增加量的IgM和/或IgG。这4例患者中的3例淋巴细胞自发产生针对麻疹和/或水痘抗原的病毒IgG抗体，表明MP在体内诱导了记忆B细胞的非特异性激活。在健康供体的淋巴细胞培养物中未发现自发的病毒抗体产生。体外血液B细胞的非特异性激活可能是由MP激活的T细胞产生的非特异性辅助因子诱导的。MP在体内也可能对B细胞有直接激活作用。
