培养气相条件对产乙酸嗜热梭菌氢化酶的影响
Effects of cultivation gas phase on hydrogenase of the acetogen Clostridium thermoaceticum.
作者信息
Kellum R, Drake H L
出版信息
J Bacteriol. 1984 Oct;160(1):466-9. doi: 10.1128/jb.160.1.466-469.1984.
Abstract
The effect of cultivation gas phase on the expression and activity of hydrogenase in heterotrophic cultures of Clostridium thermoaceticum was examined. Of the five gas phases tested, hydrogenase was maximal from cells cultivated under CO. Correlations were observed between the level of hydrogenase and the evolution of H2 by growing cultures. Activity stains of polyacrylamide gels revealed a single hydrogenase band in CO2 cells and multiple hydrogenase bands in CO cells.
摘要
研究了培养气相条件对热醋梭菌异养培养物中氢化酶表达及活性的影响。在所测试的五种气相条件中，在CO条件下培养的细胞中氢化酶活性最高。氢化酶水平与生长培养物中H2的释放之间存在相关性。聚丙烯酰胺凝胶的活性染色显示，在CO2条件下培养的细胞中有一条单一的氢化酶条带，而在CO条件下培养的细胞中有多条氢化酶条带。
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