Murase N, Takai Y, Hosaka M, Asano K, Mori M
J Oral Pathol. 1984 Oct;13(5):505-15. doi: 10.1111/j.1600-0714.1984.tb01451.x.
Complex carbohydrates in premalignant lesions of mouse submandibular gland tumors were examined by the lectin-peroxidase conjugate method. Peroxidase-conjugated lectins of PNA, RCA-1, DBA, SBA, UEA-1 and WGA were used to detect specific sugar residues of complex carbohydrates in premalignant lesions during experimental carcinogenesis. Marked reduction of PNA and SBA bindings occurred in duct-like structures and cystic lesions which were transformed from granular convoluted tubule cells. Premalignant lesions bound slightly to PNA, RCA-1, DBA, SBA and WGA and manifested increased UEA-1 binding. Squamous metaplastic epithelia of premalignant lesions manifested increased binding to PNA, RCA-1 and SBA as compared to those of duct-like structure and cystic epithelia.
采用凝集素-过氧化物酶偶联法对小鼠下颌下腺肿瘤癌前病变中的复合碳水化合物进行了检测。使用与过氧化物酶偶联的PNA、RCA-1、DBA、SBA、UEA-1和WGA凝集素来检测实验性致癌过程中癌前病变中复合碳水化合物的特定糖残基。在由颗粒曲管细胞转化而来的导管样结构和囊性病变中，PNA和SBA结合明显减少。癌前病变与PNA、RCA-1、DBA、SBA和WGA的结合较弱，而与UEA-1的结合增加。与导管样结构和囊性上皮相比，癌前病变的鳞状化生上皮与PNA、RCA-1和SBA的结合增加。
