Barr R M, Wong E, Mallet A I, Olins L A, Greaves M W
Prostaglandins. 1984 Jul;28(1):57-65. doi: 10.1016/0090-6980(84)90113-8.
Collection of exudate from suction bullae is a commonly used method for sampling human skin for mediator analysis. It is satisfactory on skin of normal structure but is unreliable on lesional psoriatic skin in which there are major structural changes and excessive scaling. Collection of exudates from abraded sites was found to be a suitable alternative method for psoriatic skin. Arachidonic acid and 12-HETE, but not PGE2, were significantly higher in exudate from abraded lesional psoriatic skin (494 +/- 88, 45.9 +/- 4.2 and 9.6 +/- 1.8 ng/ml respectively, mean +/- sem, n = 5) compared to uninvolved skin (154 + 38, 18.5 + 5.1 and 7.7 + 1.9 ng/ml) or skin of normal volunteers (119 +/- 37, 14.5 +/- 6.7 and 4.5 +/- 1.6 ng/ml, n = 7) which were similar. The coefficient of variation for exudate collection and mediator analysis was usually less than 55%. The analysis of lipoxygenase and cyclooxygenase products was simplified by the use of chlorobutane to extract preferentially arachidonic acid and HETEs from neutral aqueous solutions.
从抽吸水疱中收集渗出液是一种常用的采集人体皮肤样本以进行介质分析的方法。该方法在结构正常的皮肤上效果良好，但在病变的银屑病皮肤上不可靠，因为银屑病皮肤存在主要的结构变化和过度脱屑。发现从擦伤部位收集渗出液是银屑病皮肤的一种合适替代方法。与未受累皮肤（分别为154 + 38、18.5 + 5.1和7.7 + 1.9 ng/ml）或正常志愿者皮肤（119 +/- 37、14.5 +/- 6.7和4.5 +/- 1.6 ng/ml，n = 7）相比，擦伤的银屑病病变皮肤渗出液中的花生四烯酸和12 - HETE（但不包括PGE2）显著更高（分别为494 +/- 88、45.9 +/- 4.2和9.6 +/- 1.8 ng/ml，平均值 +/- 标准误，n = 5），未受累皮肤和正常志愿者皮肤的数值相近。渗出液收集和介质分析的变异系数通常小于55%。通过使用氯丁烷从中性水溶液中优先提取花生四烯酸和HETEs，简化了脂氧合酶和环氧化酶产物的分析。
