Kato N, Hoshino H, Harada F
Biochem Int. 1983 Nov;7(5):635-45.
The nucleotide sequence of C4 RNA, one of the "4.5S RNAs" of HeLa cells, was determined. This RNA consists of 90 nucleotides containing C-C-A at its 3'-terminus. The sequence can be drawn to form a clover-leaf structure with several unusual features and with anticodon NCA. The short term labeled molecule contains triphosphates at its 5'-terminus, whereas the mature molecule contains only monophosphate. Therefore, there must be no precursor nucleotide at the 5'-end in the primary transcript of this tRNA. Mouse cells also contain C4 RNA. Only one base exchange was observed in the extra arm in human and mouse C4 RNAs. The molecule purified from mouse liver showed serine acceptor ability.
测定了HeLa细胞“4.5S RNA”之一的C4 RNA的核苷酸序列。该RNA由90个核苷酸组成，其3'末端含有C-C-A。该序列可以绘制成具有几个不寻常特征和反密码子NCA的三叶草结构。短期标记的分子在其5'末端含有三磷酸，而成熟分子仅含有单磷酸。因此，该tRNA初级转录本的5'端一定不存在前体核苷酸。小鼠细胞也含有C4 RNA。在人和小鼠的C4 RNA的额外臂中仅观察到一个碱基交换。从小鼠肝脏中纯化的分子表现出丝氨酸接受能力。
